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2017 ; 13
(2
): 579-586
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Human serum albumin-mediated apoptin delivery suppresses breast cancer cell
growth in vitro and in vivo
#MMPMID28356932
Wu F
; Liu Y
; Li J
; Hou L
; Lei F
; Huang S
; Feng L
; Zhao X
Oncol Lett
2017[Feb]; 13
(2
): 579-586
PMID28356932
show ga
Gene therapy is one of the most promising potential therapeutic strategies for
many types of cancer. Cell apoptosis is an active, programmed physiological
process of the body, and its disruption has been closely associated with the
occurrence of tumor development. Apoptin is known to induce tumor cell apoptosis.
In the present study, the MCF-7 breast cancer cell line was transfected with a
human serum albumin (HSA) and apoptin expressing plasmid [HSA-polyethylenimine
(PEI)-pcDNA-Apoptin]. Reverse transcription-quantitative polymerase chain
reaction and western blotting were performed to detect the expression of apoptin
in the transfected MCF-7 cells, while MTT assays and flow cytometry were
conducted to detect cell viability and apoptosis. Furthermore, hematoxylin and
eosin staining was used to observe the morphology of xenografts from mice
injected with MCF-7 cells. It was demonstrated that the HSA-PEI-pcDNA-Apoptin
expression plasmid resulted in the upregulation of apoptin in MCF-7 cells, and
efficiently suppressed breast tumor growth in vivo. These findings indicated that
the use of HSA as an apoptin expression vector has potential therapeutic benefits
for cancer and confirms the requirement for the further evaluation of apoptin in
clinical trials.
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