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2017 ; 5
(5
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Pneumonia treatment by photodynamic therapy with extracorporeal illumination - an
experimental model
#MMPMID28292878
Geralde MC
; Leite IS
; Inada NM
; Salina AC
; Medeiros AI
; Kuebler WM
; Kurachi C
; Bagnato VS
Physiol Rep
2017[Mar]; 5
(5
): ä PMID28292878
show ga
Infectious pneumonia is a major cause of morbidity/mortality, mainly because of
the increasing rate of microorganisms resistant to antibiotics. Photodynamic
Therapy (PDT) is emerging as a promising approach, as effects are based on
oxidative stress, preventing microorganism resistance. In two previous studies,
the in vitro inactivation of Streptococcus pneumoniae using indocyanine green
(ICG) and infrared light source was a success killing 5 log(10) colony-forming
units (CFU/mL) with only 10 ?mol/L ICG. In this work, a proof-of-principle
protocol was designed to treat lung infections by PDT using extracorporeal
illumination with a 780 nm laser device and also ICG as photosensitizer. Hairless
mice were infected with S. pneumoniae and PDT was performed two days after
infection. For control groups, CFU recovery ranged between 10(3)-10(4)/mouse. For
PDT group, however, no bacteria were recovered in 80% of the animals. Based on
this result, animal survival was evaluated separately over 50 days. No deaths
occurred in PDT group, whereas 60% of the control group died. Our results
indicate that extracorporeal PDT has the potential for pneumonia treatment, and
pulmonary decontamination with PDT may be used as a single therapy or as an
antibiotics adjuvant.