Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28357267
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Microb+Cell
2015 ; 2
(11
): 412-428
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Electron microscopy for ultrastructural analysis and protein localization in
Saccharomyces cerevisiae
#MMPMID28357267
Frankl A
; Mari M
; Reggiori F
Microb Cell
2015[Oct]; 2
(11
): 412-428
PMID28357267
show ga
The yeast Saccharomyces cerevisiae is a key model system for studying of a
multitude of cellular processes because of its amenability to genetics, molecular
biology and biochemical procedures. Ultrastructural examinations of this
organism, though, are traditionally difficult because of the presence of a thick
cell wall and the high density of cytoplasmic proteins. A series of recent
methodological and technical developments, however, has revived interest in
morphological analyses of yeast (e.g. 123). Here we present a review of
established and new methods, from sample preparation to imaging, for the
ultrastructural analysis of S. cerevisiae. We include information for the use of
different fixation methods, embedding procedures, approaches for contrast
enhancement, and sample visualization techniques, with references to successful
examples. The goal of this review is to guide researchers that want to
investigate a particular process at the ultrastructural level in yeast by aiding
in the selection of the most appropriate approach to visualize a specific
structure or subcellular compartment.