KRAS detection on archival cytological smears by the novel fully automated
polymerase chain reaction-based Idylla mutation test
#MMPMID28331530
De Luca C
; Vigliar E
; d'Anna M
; Pisapia P
; Bellevicine C
; Malapelle U
; Troncone G
Cytojournal
2017[]; 14
(?): 5
PMID28331530
show ga
BACKGROUND: Molecular techniques are relevant to modern cytopathology, but their
implementation is difficult without molecular expertise and infrastructure. The
assessment of KRAS mutational status on cytological preparations may be useful
either to refine uncertain diagnoses on pancreatic aspirates or to yield
predictive information to plan targeted treatment of metastatic colorectal cancer
(mCRC). The novel test Idylla? enables fully automated KRAS genotyping in
approximately 2 h, even in less experienced hands. MATERIALS AND METHODS: This
study aims to validate this methodology to detect KRAS mutations on archival
cytological preparations of pancreatic cancer (n = 9) and mCRC (n = 9) by
comparing the Idylla? performance to that of standard real-time polymerase chain
reaction. RESULTS: The same 11 mutations (n = 4: p.G12D; n = 2: p.G12V; n = 2:
p.A59E/G/T; n = 1: p.G12R; n = 1: p.G13D; n = 1: p.Q61H) were detected by both
techniques. CONCLUSION: Even in less experienced laboratories, a cytopathologist
may easily integrate morphological diagnostic report with accurate KRAS mutation
detection, which is relevant for diagnostic and treatment decisions.
free
free
free
