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10.18632/oncotarget.13376 http://scihub22266oqcxt.onion/10.18632/oncotarget.13376 C5348313!5348313 !27863397     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27863397 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Oncotarget 2016 ; 7 (49 ): 80190-80207 Nephropedia Template TP {{tp|p=27863397 |t=2016. Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing |pdf=|usr=}}{{27863397 }} gab.com Text Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27863397 #FOAvip The GLI genes are transcription factors and in cancers are oncogenes, aberrantly and constitutively activated. GANT61, a specific GLI inhibitor, has induced extensive cytotoxicity in human models of colon cancer. The FOXM1 promoter was determined to be a transcriptional target of GLI1. In HT29 cells, inhibition of GLI1 binding at the GLI consensus sequence by GANT61 led to inhibited binding of Pol II, the pause-release factors DSIF, NELF and p-TEFb. The formation of R-loops (RNA:DNA hybrids, ssDNA), were reduced by GANT61 at the FOXM1 promoter. Pretreatment of HT29 cells with ?-amanitin reduced GANT61-induced ?H2AX foci. Co-localization of GLI1 and BrdU foci, inhibited by GANT61, indicated GLI1 and DNA replication to be linked. By co-immunoprecipitation and confocal microscopy, GLI1 co-localized with the DNA licensing factors ORC4, CDT1, and MCM2. Significant co-localization of GLI1 and ORC4 was inhibited by GANT61, and enrichment of ORC4 occurred at the GLI binding site in the FOXM1 promoter. CDT1 was found to be a transcription target of GLI1. Overexpression of CDT1 in HT29 and SW480 cells reduced GANT61-induced cell death, gH2AX foci, and cleavage of caspase-3. Data demonstrate involvement of transcription and of DNA replication licensing factors by non-transcriptional and transcriptional mechanisms in the GLI-dependent mechanism of action of GANT61. Twit Text FOAVip Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27863397 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27863397 #FOAvip English Wikipedia <ref>{{Cite pmid|27863397 }}</ref> Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing #MMPMID27863397 Zhang R ; Wu J ; Ferrandon S ; Glowacki KJ ; Houghton JA Oncotarget 2016[Dec]; 7 (49 ): 80190-80207 PMID27863397 show ga The GLI genes are transcription factors and in cancers are oncogenes, aberrantly and constitutively activated. GANT61, a specific GLI inhibitor, has induced extensive cytotoxicity in human models of colon cancer. The FOXM1 promoter was determined to be a transcriptional target of GLI1. In HT29 cells, inhibition of GLI1 binding at the GLI consensus sequence by GANT61 led to inhibited binding of Pol II, the pause-release factors DSIF, NELF and p-TEFb. The formation of R-loops (RNA:DNA hybrids, ssDNA), were reduced by GANT61 at the FOXM1 promoter. Pretreatment of HT29 cells with ?-amanitin reduced GANT61-induced ?H2AX foci. Co-localization of GLI1 and BrdU foci, inhibited by GANT61, indicated GLI1 and DNA replication to be linked. By co-immunoprecipitation and confocal microscopy, GLI1 co-localized with the DNA licensing factors ORC4, CDT1, and MCM2. Significant co-localization of GLI1 and ORC4 was inhibited by GANT61, and enrichment of ORC4 occurred at the GLI binding site in the FOXM1 promoter. CDT1 was found to be a transcription target of GLI1. Overexpression of CDT1 in HT29 and SW480 cells reduced GANT61-induced cell death, gH2AX foci, and cleavage of caspase-3. Data demonstrate involvement of transcription and of DNA replication licensing factors by non-transcriptional and transcriptional mechanisms in the GLI-dependent mechanism of action of GANT61. |Antineoplastic Agents/*pharmacology [MESH] |Binding Sites [MESH] |Cell Cycle Proteins/genetics/metabolism [MESH] |Cell Death/drug effects [MESH] |Colonic Neoplasms/*drug therapy/genetics/metabolism/pathology [MESH] |DNA Replication/*drug effects [MESH] |Dose-Response Relationship, Drug [MESH] |Forkhead Box Protein M1/genetics/metabolism [MESH] |Gene Expression Regulation, Neoplastic/*drug effects [MESH] |HT29 Cells [MESH] |Histones/metabolism [MESH] |Humans [MESH] |Minichromosome Maintenance Complex Component 2/genetics/metabolism [MESH] |Nuclear Proteins/genetics/metabolism [MESH] |Origin Recognition Complex/genetics/metabolism [MESH] |Promoter Regions, Genetic [MESH] |Pyridines/*pharmacology [MESH] |Pyrimidines/*pharmacology [MESH] |RNA Polymerase II/genetics/metabolism [MESH] |Transcription Factors/genetics/metabolism [MESH] |Transcription, Genetic/*drug effects [MESH] |Transcriptional Elongation Factors/genetics/metabolism [MESH] |Transfection [MESH]Targeting GLI by GANT61 involves mechanisms dependent on inhibition of(epmc).pdf DeepDyve Pubget Overpricing