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10.18632/oncotarget.13110 http://scihub22266oqcxt.onion/10.18632/oncotarget.13110 C5347777!5347777 !27825135     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27825135 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Oncotarget 2016 ; 7 (50 ): 83392-83408 Nephropedia Template TP {{tp|p=27825135 |t=2016. CD19-specific triplebody SPM-1 engages NK and ?? T cells for rapid and efficient lysis of malignant B-lymphoid cells |pdf=|usr=}}{{27825135 }} gab.com Text CD19-specific triplebody SPM-1 engages NK and T cells for rapid and efficient lysis of malignant B-lymphoid cells JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27825135 #FOAvip Triplebodies are antibody-derived recombinant proteins carrying 3 antigen-binding domains in a single polypeptide chain. Triplebody SPM-1 was designed for lysis of CD19-bearing malignant B-lymphoid cells through the engagement of CD16-expressing cytolytic effectors, including NK and ?? T cells.SPM-1 is an optimized version of triplebody ds(19-16-19) and includes humanization, disulfide stabilization and the removal of potentially immunogenic sequences. A three-step chromatographic procedure yielded 1.7 - 5.5 mg of purified, monomeric protein per liter of culture medium. In cytolysis assays with NK cell effectors, SPM-1 mediated potent lysis of cancer-derived B cell lines and primary cells from patients with various B-lymphoid malignancies, which surpassed the ADCC activity of the therapeutic antibody Rituximab. EC50-values ranged from 3 to 86 pM. Finally, in an impedance-based assay, SPM-1 mediated a particularly rapid lysis of CD19-bearing target cells by engaging and activating both primary and expanded human ?? T cells from healthy donors as effectors.These data establish SPM-1 as a useful tool for a kinetic analysis of the cytolytic reactions mediated by ?? T and NK cells and as an agent deserving further development towards clinical use for the treatment of B-lymphoid malignancies. Twit Text FOAVip CD19-specific triplebody SPM-1 engages NK and T cells for rapid and efficient lysis of malignant B-lymphoid cells ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27825135 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27825135 #FOAvip English Wikipedia <ref>{{Cite pmid|27825135 }}</ref> CD19-specific triplebody SPM-1 engages NK and ?? T cells for rapid and efficient lysis of malignant B-lymphoid cells #MMPMID27825135 Schiller CB ; Braciak TA ; Fenn NC ; Seidel UJ ; Roskopf CC ; Wildenhain S ; Honegger A ; Schubert IA ; Schele A ; Lämmermann K ; Fey GH ; Jacob U ; Lang P ; Hopfner KP ; Oduncu FS Oncotarget 2016[Dec]; 7 (50 ): 83392-83408 PMID27825135 show ga Triplebodies are antibody-derived recombinant proteins carrying 3 antigen-binding domains in a single polypeptide chain. Triplebody SPM-1 was designed for lysis of CD19-bearing malignant B-lymphoid cells through the engagement of CD16-expressing cytolytic effectors, including NK and ?? T cells.SPM-1 is an optimized version of triplebody ds(19-16-19) and includes humanization, disulfide stabilization and the removal of potentially immunogenic sequences. A three-step chromatographic procedure yielded 1.7 - 5.5 mg of purified, monomeric protein per liter of culture medium. In cytolysis assays with NK cell effectors, SPM-1 mediated potent lysis of cancer-derived B cell lines and primary cells from patients with various B-lymphoid malignancies, which surpassed the ADCC activity of the therapeutic antibody Rituximab. EC50-values ranged from 3 to 86 pM. Finally, in an impedance-based assay, SPM-1 mediated a particularly rapid lysis of CD19-bearing target cells by engaging and activating both primary and expanded human ?? T cells from healthy donors as effectors.These data establish SPM-1 as a useful tool for a kinetic analysis of the cytolytic reactions mediated by ?? T and NK cells and as an agent deserving further development towards clinical use for the treatment of B-lymphoid malignancies. |Antigens, CD19/*immunology [MESH] |Antineoplastic Agents, Immunological/immunology/*pharmacology [MESH] |Cell Line, Tumor [MESH] |Cell Proliferation/drug effects [MESH] |Cytotoxicity, Immunologic/*drug effects [MESH] |Dose-Response Relationship, Drug [MESH] |Humans [MESH] |Intraepithelial Lymphocytes/*drug effects/immunology [MESH] |Killer Cells, Natural/*drug effects/immunology [MESH] |Kinetics [MESH] |Lymphocyte Activation/drug effects [MESH] |Lymphocytes, Tumor-Infiltrating/*drug effects/immunology [MESH] |Lymphoma, B-Cell/*drug therapy/immunology/pathology [MESH] |Rituximab/pharmacology [MESH]CD19-specific triplebody SPM-1 engages NK and ?? T cells for rapid and(epmc).pdf DeepDyve Pubget Overpricing