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Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring #MMPMID28223521
Cao Y; Hjort M; Chen H; Birey F; Leal-Ortiz SA; Han CM; Santiago JG; Pa?ca SP; Wu JC; Melosh NA
Proc Natl Acad Sci U S A 2017[Mar]; 114 (10): E1866-74 PMID28223521show ga
Cell content analysis has rapidly become one of the most important new tools for measuring cell phenotype and behavior. However, the central limitation of current sampling technologies is they are destructive and must lyse the cells to measure the contents. This destruction prevents knowledge of prior or future states of the cell, which is particularly important for dynamic cell processes, such as development and differentiation. Here, we show a nondestructive longitudinal sampling and analysis platform that can sample repeatedly and accurately from the same single cell or group of cells over a long time period. We demonstrate sampling of both proteins and mRNA for cell lines as well as human-derived cardiomyocytes and astrocytes.