Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1186/s12931-017-0529-6 http://scihub22266oqcxt.onion/10.1186/s12931-017-0529-6 C5346259!5346259!28284212     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Respir+Res 2017 ; 18 (ä): ä Nephropedia Template TP {{tp|p=28284212|t=2017. Combination of glycopyrronium and indacaterol inhibits carbachol-induced ERK5 signal in fibrotic processes |pdf=|usr=}}{{28284212}} gab.com Text Combination of glycopyrronium and indacaterol inhibits carbachol-induced ERK5 signal in fibrotic processes JO: Respir Res http://www.kidney.de/pget.php?&tw=1&pmid=28284212 #FOAvip Background: Airway fibrosis is one of the pathological features of chronic obstructive pulmonary disease (COPD), and recent studies revealed that acetylcholine plays an important role in the development of airway remodeling by stimulating proliferation and collagen synthesis of lung fibroblasts. This study was designed to examine the effects of a long-acting muscarinic receptor antagonist (LAMA) glycopyrronium and a long-acting ?2 adrenergic receptor agonist (LABA) indacaterol on acetylcholine-mediated fibrotic responses in lung fibroblasts. Methods: After carbachol (CCh) or transforming growth factor-?1 (TGF-?1) exposure, the response to glycopyrronium and indacaterol was determined in vitro in fibroblasts isolated from mild-to-moderate COPD lung tissue. The ability of fibroblasts to mediate the contraction of collagen gels was assessed. The expression of ?-smooth muscle actin (?-SMA) and the phosphorylation of extracellular-signal-regulated kinase 5 (ERK5) were determined by immunoblot. TGF-?1 was quantified by ELISA and acetylcholine was quantified by liquid chromatography tandem-mass spectrometry. Results: CCh stimulated fibroblast-mediated collagen gel contraction and ?-SMA expression and TGF-?1 release by fibroblasts. Blockade of autocrine TGF-?1 attenuated CCh-mediated fibrotic responses, while TGF-?1 did not stimulate acetylcholine release. Glycopyrronium plus indacaterol significantly attenuated CCh- and TGF-?1-mediated fibrotic responses through inhibition of ERK5 phosphorylation. Notably, the magnitudes of CCh- and TGF-?1-stimulated gel contraction, CCh-induced TGF-?1 release, and ERK5 phosphorylation were greater in fibroblasts isolated from COPD subjects than in those from non-smokers. Conclusions: CCh induced TGF-?1 self-sustaining signaling loops by potentiating ERK5 signaling and promoted myofibroblast activity. This autocrine signaling mechanism may be an attractive therapeutic target to block the fibrotic response, which was modulated by the combination of glycopyrronium and indacaterol. Twit Text FOAVip Combination of glycopyrronium and indacaterol inhibits carbachol-induced ERK5 signal in fibrotic processes ????Respir Res http://www.kidney.de/pget.php?&tw=1&pmid=28284212 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28284212 #FOAvip English Wikipedia <ref>{{Cite pmid|28284212}}</ref> Combination of glycopyrronium and indacaterol inhibits carbachol-induced ERK5 signal in fibrotic processes #MMPMID28284212 Namba Y; Togo S; Tulafu M; Kadoya K; Nagahama KY; Taka H; Kaga N; Orimo A; Liu X; Takahashi K Respir Res 2017[]; 18 (ä): ä PMID28284212show ga Background: Airway fibrosis is one of the pathological features of chronic obstructive pulmonary disease (COPD), and recent studies revealed that acetylcholine plays an important role in the development of airway remodeling by stimulating proliferation and collagen synthesis of lung fibroblasts. This study was designed to examine the effects of a long-acting muscarinic receptor antagonist (LAMA) glycopyrronium and a long-acting ?2 adrenergic receptor agonist (LABA) indacaterol on acetylcholine-mediated fibrotic responses in lung fibroblasts. Methods: After carbachol (CCh) or transforming growth factor-?1 (TGF-?1) exposure, the response to glycopyrronium and indacaterol was determined in vitro in fibroblasts isolated from mild-to-moderate COPD lung tissue. The ability of fibroblasts to mediate the contraction of collagen gels was assessed. The expression of ?-smooth muscle actin (?-SMA) and the phosphorylation of extracellular-signal-regulated kinase 5 (ERK5) were determined by immunoblot. TGF-?1 was quantified by ELISA and acetylcholine was quantified by liquid chromatography tandem-mass spectrometry. Results: CCh stimulated fibroblast-mediated collagen gel contraction and ?-SMA expression and TGF-?1 release by fibroblasts. Blockade of autocrine TGF-?1 attenuated CCh-mediated fibrotic responses, while TGF-?1 did not stimulate acetylcholine release. Glycopyrronium plus indacaterol significantly attenuated CCh- and TGF-?1-mediated fibrotic responses through inhibition of ERK5 phosphorylation. Notably, the magnitudes of CCh- and TGF-?1-stimulated gel contraction, CCh-induced TGF-?1 release, and ERK5 phosphorylation were greater in fibroblasts isolated from COPD subjects than in those from non-smokers. Conclusions: CCh induced TGF-?1 self-sustaining signaling loops by potentiating ERK5 signaling and promoted myofibroblast activity. This autocrine signaling mechanism may be an attractive therapeutic target to block the fibrotic response, which was modulated by the combination of glycopyrronium and indacaterol. äCombination of glycopyrronium and indacaterol inhibits carbachol-induc(epmc).pdf DeepDyve Pubget Overpricing