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10.7171/jbt.17-2801-004 http://scihub22266oqcxt.onion/10.7171/jbt.17-2801-004 C5345951!5345951 !28337070     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28337070 &cmd=llinks): Failed to open stream: HTTP request failed! 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Genomic Methods and Microbiological Technologies for Profiling Novel and Extreme Environments for the Extreme Microbiome Project (XMP) |pdf=|usr=}}{{28337070 }} gab.com Text Genomic Methods and Microbiological Technologies for Profiling Novel and Extreme Environments for the Extreme Microbiome Project (XMP) JO: J Biomol Tech http://www.kidney.de/pget.php?&tw=1&pmid=28337070 #FOAvip The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques. The goals are multifaceted, including development and refinement of new techniques for the following: 1) the detection and characterization of novel microbes, 2) the evaluation of nucleic acid techniques for extremophilic samples, and 3) the identification and implementation of the appropriate bioinformatics pipelines. Here, we highlight the different ongoing projects that we have been working on, as well as details on the various methods we use to characterize the microbiome and metagenome of these complex samples. In particular, we present data of a novel multienzyme extraction protocol that we developed, called Polyzyme or MetaPolyZyme. Presently, the XMP is characterizing sample sites around the world with the intent of discovering new species, genes, and gene clusters. Once a project site is complete, the resulting data will be publically available. Sites include Lake Hillier in Western Australia, the "Door to Hell" crater in Turkmenistan, deep ocean brine lakes of the Gulf of Mexico, deep ocean sediments from Greenland, permafrost tunnels in Alaska, ancient microbial biofilms from Antarctica, Blue Lagoon Iceland, Ethiopian toxic hot springs, and the acidic hypersaline ponds in Western Australia. Twit Text FOAVip Genomic Methods and Microbiological Technologies for Profiling Novel and Extreme Environments for the Extreme Microbiome Project (XMP) ????J Biomol Tech http://www.kidney.de/pget.php?&tw=1&pmid=28337070 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28337070 #FOAvip English Wikipedia <ref>{{Cite pmid|28337070 }}</ref> Genomic Methods and Microbiological Technologies for Profiling Novel and Extreme Environments for the Extreme Microbiome Project (XMP) #MMPMID28337070 Tighe S ; Afshinnekoo E ; Rock TM ; McGrath K ; Alexander N ; McIntyre A ; Ahsanuddin S ; Bezdan D ; Green SJ ; Joye S ; Stewart Johnson S ; Baldwin DA ; Bivens N ; Ajami N ; Carmical JR ; Herriott IC ; Colwell R ; Donia M ; Foox J ; Greenfield N ; Hunter T ; Hoffman J ; Hyman J ; Jorgensen E ; Krawczyk D ; Lee J ; Levy S ; Garcia-Reyero N ; Settles M ; Thomas K ; Gómez F ; Schriml L ; Kyrpides N ; Zaikova E ; Penterman J ; Mason CE J Biomol Tech 2017[Apr]; 28 (1 ): 31-39 PMID28337070 show ga The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques. The goals are multifaceted, including development and refinement of new techniques for the following: 1) the detection and characterization of novel microbes, 2) the evaluation of nucleic acid techniques for extremophilic samples, and 3) the identification and implementation of the appropriate bioinformatics pipelines. Here, we highlight the different ongoing projects that we have been working on, as well as details on the various methods we use to characterize the microbiome and metagenome of these complex samples. In particular, we present data of a novel multienzyme extraction protocol that we developed, called Polyzyme or MetaPolyZyme. Presently, the XMP is characterizing sample sites around the world with the intent of discovering new species, genes, and gene clusters. Once a project site is complete, the resulting data will be publically available. Sites include Lake Hillier in Western Australia, the "Door to Hell" crater in Turkmenistan, deep ocean brine lakes of the Gulf of Mexico, deep ocean sediments from Greenland, permafrost tunnels in Alaska, ancient microbial biofilms from Antarctica, Blue Lagoon Iceland, Ethiopian toxic hot springs, and the acidic hypersaline ponds in Western Australia. |*Environmental Microbiology [MESH] |DNA, Bacterial/genetics/isolation & purification [MESH] |Extreme Environments [MESH] |Metagenome [MESH] |Microbiota/*genetics [MESH] |Molecular Typing/standards [MESH] |RNA, Bacterial/genetics/isolation & purification [MESH] |Reference Standards [MESH]Genomic Methods and Microbiological Technologies for Profiling Novel a(epmc).pdf DeepDyve Pubget Overpricing