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10.1186/s13287-017-0495-4 http://scihub22266oqcxt.onion/10.1186/s13287-017-0495-4 C5345162!5345162 !28279199     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28279199 &cmd=llinks): Failed to open stream: HTTP request failed! 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CD117(+)CD44(+) Stem T Cells Develop in the Thymus and Potently Suppress T-cell Proliferation by Modulating the CTLA-4 Pathway |pdf=|usr=}}{{28279199 }} gab.com Text CD117(+)CD44(+) Stem T Cells Develop in the Thymus and Potently Suppress T-cell Proliferation by Modulating the CTLA-4 Pathway JO: Stem Cell Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=28279199 #FOAvip BACKGROUND: CD117 is expressed on double-negative (DN; CD4(-)CD8(-)) cells (Nat Rev Immunol 14:529-545; 2014), but whether it is expressed in other stages and its subsequent functions are unclear. We used an improved method of flow cytometry to analyze different populations of thymocytes (Sci Rep 4:5781; 2014). The expression of CD117 and CTLA-4 were directly assayed in the early stage of thymocytes. METHODS: Flow cytometry was used to analyze different populations of thymocytes, and T-cell proliferation assays, RT-PCR, and real-time RT-PCR were used to characterize the stem cells and examine the function of CD44(+)CD117(+) cells. RESULTS: In DN cells, CD117 expression was greatest on CD44(+)CD25(+) cells (DN(2)), followed by CD44(+)CD25(-) (DN(1)), CD44(-)CD25(+) (DN(3)), and CD44(-)CD25(-) (DN(4)) cells. In thymocytes, CD117 expression was highest in DN cells, followed by single-positive (SP; CD4 or CD8) and double-positive (DP; CD4(+)CD8(+)) cells.  Especially, CD117 expression was positively associated with CD44 and CTLA-4 expression. CTLA-4 expression was highest in DN cells, followed by SP and DP cells. CTLA-4 expression was positively associated with CD25, CD44, and Foxp3 expression. CD44(+)CD117(+) T cells expressed more CTLA-4, which suppressed T-cell proliferation and blocked CTLA-4 to cause antibody-induced T-cell proliferation. CONCLUSION: These results suggest that CD44(+)CD117(+) T cells are stem cells and a specific T-cell phenotype that initially develops in the thymus, but they do not progress through DN(3) and DN(4) stages, lack a DP stage, and potently suppress T-cell proliferation and modulate the CTLA-4 pathway. Twit Text FOAVip CD117(+)CD44(+) Stem T Cells Develop in the Thymus and Potently Suppress T-cell Proliferation by Modulating the CTLA-4 Pathway ????Stem Cell Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=28279199 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28279199 #FOAvip English Wikipedia <ref>{{Cite pmid|28279199 }}</ref> CD117(+)CD44(+) Stem T Cells Develop in the Thymus and Potently Suppress T-cell Proliferation by Modulating the CTLA-4 Pathway #MMPMID28279199 Wei Y ; Hu Z ; Gu W ; Liu G ; Shi B ; Liu E ; Liu T Stem Cell Res Ther 2017[Mar]; 8 (1 ): 56 PMID28279199 show ga BACKGROUND: CD117 is expressed on double-negative (DN; CD4(-)CD8(-)) cells (Nat Rev Immunol 14:529-545; 2014), but whether it is expressed in other stages and its subsequent functions are unclear. We used an improved method of flow cytometry to analyze different populations of thymocytes (Sci Rep 4:5781; 2014). The expression of CD117 and CTLA-4 were directly assayed in the early stage of thymocytes. METHODS: Flow cytometry was used to analyze different populations of thymocytes, and T-cell proliferation assays, RT-PCR, and real-time RT-PCR were used to characterize the stem cells and examine the function of CD44(+)CD117(+) cells. RESULTS: In DN cells, CD117 expression was greatest on CD44(+)CD25(+) cells (DN(2)), followed by CD44(+)CD25(-) (DN(1)), CD44(-)CD25(+) (DN(3)), and CD44(-)CD25(-) (DN(4)) cells. In thymocytes, CD117 expression was highest in DN cells, followed by single-positive (SP; CD4 or CD8) and double-positive (DP; CD4(+)CD8(+)) cells.  Especially, CD117 expression was positively associated with CD44 and CTLA-4 expression. CTLA-4 expression was highest in DN cells, followed by SP and DP cells. CTLA-4 expression was positively associated with CD25, CD44, and Foxp3 expression. CD44(+)CD117(+) T cells expressed more CTLA-4, which suppressed T-cell proliferation and blocked CTLA-4 to cause antibody-induced T-cell proliferation. CONCLUSION: These results suggest that CD44(+)CD117(+) T cells are stem cells and a specific T-cell phenotype that initially develops in the thymus, but they do not progress through DN(3) and DN(4) stages, lack a DP stage, and potently suppress T-cell proliferation and modulate the CTLA-4 pathway. |Animals [MESH] |CTLA-4 Antigen/genetics/*immunology [MESH] |Cell Differentiation/genetics [MESH] |Cell Proliferation/genetics [MESH] |Flow Cytometry [MESH] |Humans [MESH] |Hyaluronan Receptors/genetics/*immunology [MESH] |Lymphocyte Activation/immunology [MESH] |Mice [MESH] |Proto-Oncogene Proteins c-kit/genetics/*immunology [MESH] |Signal Transduction [MESH] |Stem Cells/cytology/*immunology/metabolism [MESH] |T-Lymphocyte Subsets/immunology [MESH]CD117(+)CD44(+) Stem T Cells Develop in the Thymus and Potently Suppre(epmc).pdf DeepDyve Pubget Overpricing