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10.1038/srep44277 http://scihub22266oqcxt.onion/10.1038/srep44277 C5345070!5345070!28281674     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28281674|t=2017. New insights into the intracellular distribution pattern of cationic amphiphilic drugs |pdf=|usr=}}{{28281674}} gab.com Text New insights into the intracellular distribution pattern of cationic amphiphilic drugs JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28281674 #FOAvip Cationic amphiphilic drugs (CADs) comprise a wide variety of different substance classes such as antidepressants, antipsychotics, and antiarrhythmics. It is well recognized that CADs accumulate in certain intracellular compartments leading to specific morphological changes of cells. So far, no adequate technique exists allowing for ultrastructural analysis of CAD in intact cells. Azidobupramine, a recently described multifunctional antidepressant analogue, allows for the first time to perform high-resolution studies of CADs on distribution pattern and morphological changes in intact cells. We showed here that the intracellular distribution pattern of azidobupramine strongly depends on drug concentration and exposure time. The mitochondrial compartment (mDsRed) and the late endo-lysosomal compartment (CD63-GFP) were the preferred localization sites at low to intermediate concentrations (i.e. 1??M, 5??M). In contrast, the autophagosomal compartment (LC3-GFP) can only be reached at high concentrations (10??M) and long exposure times (72?hrs). At the morphological level, LC3-clustering became only prominent at high concentrations (10??M), while changes in CD63 pattern already occurred at intermediate concentrations (5??M). To our knowledge, this is the first study that establishes a link between intracellular CAD distribution pattern and morphological changes. Therewith, our results allow for gaining deeper understanding of intracellular effects of CADs. Twit Text FOAVip New insights into the intracellular distribution pattern of cationic amphiphilic drugs ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28281674 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28281674 #FOAvip English Wikipedia <ref>{{Cite pmid|28281674}}</ref> New insights into the intracellular distribution pattern of cationic amphiphilic drugs #MMPMID28281674 Vater M; Möckl L; Gormanns V; Schultz Fademrecht C; Mallmann AM; Ziegart-Sadowska K; Zaba M; Frevert ML; Bräuchle C; Holsboer F; Rein T; Schmidt U; Kirmeier T Sci Rep 2017[]; 7 (ä): ä PMID28281674show ga Cationic amphiphilic drugs (CADs) comprise a wide variety of different substance classes such as antidepressants, antipsychotics, and antiarrhythmics. It is well recognized that CADs accumulate in certain intracellular compartments leading to specific morphological changes of cells. So far, no adequate technique exists allowing for ultrastructural analysis of CAD in intact cells. Azidobupramine, a recently described multifunctional antidepressant analogue, allows for the first time to perform high-resolution studies of CADs on distribution pattern and morphological changes in intact cells. We showed here that the intracellular distribution pattern of azidobupramine strongly depends on drug concentration and exposure time. The mitochondrial compartment (mDsRed) and the late endo-lysosomal compartment (CD63-GFP) were the preferred localization sites at low to intermediate concentrations (i.e. 1??M, 5??M). In contrast, the autophagosomal compartment (LC3-GFP) can only be reached at high concentrations (10??M) and long exposure times (72?hrs). At the morphological level, LC3-clustering became only prominent at high concentrations (10??M), while changes in CD63 pattern already occurred at intermediate concentrations (5??M). To our knowledge, this is the first study that establishes a link between intracellular CAD distribution pattern and morphological changes. Therewith, our results allow for gaining deeper understanding of intracellular effects of CADs. äNew insights into the intracellular distribution pattern of cationic a(epmc).pdf DeepDyve Pubget Overpricing