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10.1111/cei.12904 http://scihub22266oqcxt.onion/10.1111/cei.12904 C5343369!5343369 !27880974     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 276.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 276.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27880974 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Clin+Exp+Immunol 2017 ; 188 (1 ): 12-21 Nephropedia Template TP {{tp|p=27880974 |t=2017. Deep sequencing of the TCR-? repertoire of human forkhead box protein 3 (FoxP3)(+) and FoxP3(-) T cells suggests that they are completely distinct and non-overlapping |pdf=|usr=}}{{27880974 }} gab.com Text Deep sequencing of the TCR- repertoire of human forkhead box protein 3 (FoxP3)(+) and FoxP3(-) T cells suggests that they are completely distinct and non-overlapping JO: Clin Exp Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27880974 #FOAvip Maintenance of peripheral tolerance requires a balance between autoreactive conventional T cells (T(conv) ) and thymically derived forkhead box protein 3 (FoxP3)(+) regulatory T cells (tT(regs) ). Considerable controversy exists regarding the similarities/differences in T cell receptor (TCR) repertoires expressed by T(conv) and tT(regs) . We generated highly purified populations of human adult and cord blood T(conv) and tT(regs) based on the differential expression of CD25 and CD127. The purity of the sorted populations was validated by intracellular staining for FoxP3 and Helios. We also purified an overlap group of CD4 T cells from adult donors to ensure that considerable numbers of shared clonotypes could be detected when present. We used deep sequencing of entire TCR-? CDR3 sequences to analyse the TCR repertoire of T(conv) and tT(regs) . Our studies suggest that both neonatal and adult human T(conv) and tT(reg) cells are, in fact, entirely distinct CD4 T cell lineages. Twit Text FOAVip Deep sequencing of the TCR- repertoire of human forkhead box protein 3 (FoxP3)(+) and FoxP3(-) T cells suggests that they are completely distinct and non-overlapping ????Clin Exp Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27880974 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27880974 #FOAvip English Wikipedia <ref>{{Cite pmid|27880974 }}</ref> Deep sequencing of the TCR-? repertoire of human forkhead box protein 3 (FoxP3)(+) and FoxP3(-) T cells suggests that they are completely distinct and non-overlapping #MMPMID27880974 Golding A ; Darko S ; Wylie WH ; Douek DC ; Shevach EM Clin Exp Immunol 2017[Apr]; 188 (1 ): 12-21 PMID27880974 show ga Maintenance of peripheral tolerance requires a balance between autoreactive conventional T cells (T(conv) ) and thymically derived forkhead box protein 3 (FoxP3)(+) regulatory T cells (tT(regs) ). Considerable controversy exists regarding the similarities/differences in T cell receptor (TCR) repertoires expressed by T(conv) and tT(regs) . We generated highly purified populations of human adult and cord blood T(conv) and tT(regs) based on the differential expression of CD25 and CD127. The purity of the sorted populations was validated by intracellular staining for FoxP3 and Helios. We also purified an overlap group of CD4 T cells from adult donors to ensure that considerable numbers of shared clonotypes could be detected when present. We used deep sequencing of entire TCR-? CDR3 sequences to analyse the TCR repertoire of T(conv) and tT(regs) . Our studies suggest that both neonatal and adult human T(conv) and tT(reg) cells are, in fact, entirely distinct CD4 T cell lineages. |*High-Throughput Nucleotide Sequencing [MESH] |*Phenotype [MESH] |Biomarkers [MESH] |Clonal Evolution [MESH] |Complementarity Determining Regions/genetics [MESH] |Forkhead Transcription Factors/*metabolism [MESH] |Humans [MESH] |Immunophenotyping [MESH] |Receptors, Antigen, T-Cell, alpha-beta/*genetics/metabolism [MESH] |T-Lymphocyte Subsets/immunology/*metabolism [MESH] |T-Lymphocytes, Regulatory/immunology/metabolism [MESH]Deep sequencing of the TCR-? repertoire of human forkhead box protein (epmc).pdf DeepDyve Pubget Overpricing