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10.18632/oncotarget.12225 http://scihub22266oqcxt.onion/10.18632/oncotarget.12225 C5342592!5342592 !27683125     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27683125 &cmd=llinks): Failed to open stream: HTTP request failed! 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Reduced argininosuccinate synthetase expression in refractory sarcomas: Impacts on therapeutic potential and drug resistance |pdf=|usr=}}{{27683125 }} gab.com Text Reduced argininosuccinate synthetase expression in refractory sarcomas: Impacts on therapeutic potential and drug resistance JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27683125 #FOAvip BACKGROUND: Treating drug-resistant sarcomas remains a major challenge. The present study aimed to identify a novel therapy for drug-resistant sarcomas based on metabolic errors involving argininosuccinate synthetase1 (ASS1). RESULTS: ASS1 expression was reduced in Dox-resistant sarcoma cells. Immunohistochemistry and real-time PCR showed an inverse correlation between ASS1 and P-gp expressions. The inhibition of cellular proliferation with G1-arrest was shown to lead to autophagy with arginine deprivation. In addition, the combination of an autophagy inhibitor plus arginine deprivation was more effective than arginine deprivation alone. In cells with suppressed ASS1 expression, P-gp expression was upregulated as compared to that in negative controls. DISCUSSION: These results indicate that the reduced ASS1 expression in Dox-resistant sarcomas may contribute to drug resistance in association with the expression of P-gp. ASS1 deficiency is a potential target for novel drug therapies. The combination of arginine-deprivation therapy and an autophagy inhibitor may have anti-tumor effects in refractory sarcomas. METHODS: We assessed the expressions of ASS1 and P-glycoprotein (P-gp) in clinical specimens and cell lines of osteosarcoma (KHOS), doxorubicin (Dox)-resistant osteosarcoma (KHOSR2), epithelioid sarcomas (ES-X and VAESBJ) and alveolar soft part sarcoma (ASPS-KY). Each cell line was cultured in arginine-containing and arginine-free media. Cell growth was assessed using an XTT assay and flow cytometry. We analyzed the induction of autophagy in arginine-free medium. Moreover, we assessed the expression of P-gp after suppressing ASS1 in Dox-sensitive cells (MCF-7 and KHOS) and after transfecting ASS1 into Dox-resistant cells (ES-X, VAESBJ, ASPS-KY and KHOSR2). Twit Text FOAVip Reduced argininosuccinate synthetase expression in refractory sarcomas: Impacts on therapeutic potential and drug resistance ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27683125 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27683125 #FOAvip English Wikipedia <ref>{{Cite pmid|27683125 }}</ref> Reduced argininosuccinate synthetase expression in refractory sarcomas: Impacts on therapeutic potential and drug resistance #MMPMID27683125 Kim Y ; Kobayashi E ; Kubota D ; Suehara Y ; Mukaihara K ; Akaike K ; Ito A ; Kaneko K ; Chuman H ; Kawai A ; Kitano S Oncotarget 2016[Oct]; 7 (43 ): 70832-70844 PMID27683125 show ga BACKGROUND: Treating drug-resistant sarcomas remains a major challenge. The present study aimed to identify a novel therapy for drug-resistant sarcomas based on metabolic errors involving argininosuccinate synthetase1 (ASS1). RESULTS: ASS1 expression was reduced in Dox-resistant sarcoma cells. Immunohistochemistry and real-time PCR showed an inverse correlation between ASS1 and P-gp expressions. The inhibition of cellular proliferation with G1-arrest was shown to lead to autophagy with arginine deprivation. In addition, the combination of an autophagy inhibitor plus arginine deprivation was more effective than arginine deprivation alone. In cells with suppressed ASS1 expression, P-gp expression was upregulated as compared to that in negative controls. DISCUSSION: These results indicate that the reduced ASS1 expression in Dox-resistant sarcomas may contribute to drug resistance in association with the expression of P-gp. ASS1 deficiency is a potential target for novel drug therapies. The combination of arginine-deprivation therapy and an autophagy inhibitor may have anti-tumor effects in refractory sarcomas. METHODS: We assessed the expressions of ASS1 and P-glycoprotein (P-gp) in clinical specimens and cell lines of osteosarcoma (KHOS), doxorubicin (Dox)-resistant osteosarcoma (KHOSR2), epithelioid sarcomas (ES-X and VAESBJ) and alveolar soft part sarcoma (ASPS-KY). Each cell line was cultured in arginine-containing and arginine-free media. Cell growth was assessed using an XTT assay and flow cytometry. We analyzed the induction of autophagy in arginine-free medium. Moreover, we assessed the expression of P-gp after suppressing ASS1 in Dox-sensitive cells (MCF-7 and KHOS) and after transfecting ASS1 into Dox-resistant cells (ES-X, VAESBJ, ASPS-KY and KHOSR2). |*Drug Resistance, Neoplasm [MESH] |ATP Binding Cassette Transporter, Subfamily B/metabolism [MESH] |Adolescent [MESH] |Adult [MESH] |Aged [MESH] |Antibiotics, Antineoplastic/pharmacology/therapeutic use [MESH] |Arginine/metabolism [MESH] |Argininosuccinate Synthase/genetics/*metabolism [MESH] |Autophagy/drug effects [MESH] |Cell Line, Tumor [MESH] |Cell Proliferation/drug effects [MESH] |Cell Separation/methods [MESH] |Child [MESH] |Down-Regulation [MESH] |Doxorubicin/pharmacology/therapeutic use [MESH] |Female [MESH] |Flow Cytometry [MESH] |G1 Phase Cell Cycle Checkpoints [MESH] |Humans [MESH] |Immunohistochemistry [MESH] |Male [MESH] |Middle Aged [MESH] |Neoplasm Recurrence, Local/*enzymology/pathology [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Sarcoma/drug therapy/*enzymology/pathology [MESH]Reduced argininosuccinate synthetase expression in refractory sarcomas(epmc).pdf DeepDyve Pubget Overpricing