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10.18632/oncotarget.11956 http://scihub22266oqcxt.onion/10.18632/oncotarget.11956 C5342475!5342475 !27626491     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27626491 &cmd=llinks): Failed to open stream: HTTP request failed! 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Delineation of the central melanocortin circuitry controlling the kidneys by a virally mediated transsynaptic tracing study in transgenic mouse model |pdf=|usr=}}{{27626491 }} gab.com Text Delineation of the central melanocortin circuitry controlling the kidneys by a virally mediated transsynaptic tracing study in transgenic mouse model JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27626491 #FOAvip To examine if brain neurons involved in the efferent control of the kidneys possess melanocortin-4 receptor (MC4-R) and/or tryptophan hydroxylase (TPH). Retrograde tracing pseudorabies virus (PRV)-614 was injected into the kidneys in adult male MC4R-green fluorescent protein (GFP) transgenic mice. After a survival time of 3-7 days, spinal cord and brain were removed and sectioned, and processed for PRV-614 visualization. The neurochemical phenotype of PRV-614-positive neurons was identified using double or triple immunocytochemical labeling against PRV-614, MC4R, or TPH. Double and triple labeling was quantified using microscopy. The majority of PRV-614 immunopositive neurons which also expressed immunoreactivity for MC4R were located in the ipsilateral intermediolateral cell column (IML) of the thoracic spinal cord, the paraventricular nucleus (PVN) of the hypothalamus, and raphe pallidus (RPa), nucleus raphe magnus (NRM) and ventromedial medulla (VMM) of the brainstem. Triple-labeled MC4R/PRV-614/TPH neurons were concentrated in the PVN, RPa, NRM and VMM. These data strongly suggest that central MC4R and TPH are involved in the efferent neuronal control of the kidneys. Twit Text FOAVip Delineation of the central melanocortin circuitry controlling the kidneys by a virally mediated transsynaptic tracing study in transgenic mouse model ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27626491 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27626491 #FOAvip English Wikipedia <ref>{{Cite pmid|27626491 }}</ref> Delineation of the central melanocortin circuitry controlling the kidneys by a virally mediated transsynaptic tracing study in transgenic mouse model #MMPMID27626491 Liu TT ; Liu BW ; He ZG ; Feng L ; Liu SG ; Xiang HB Oncotarget 2016[Oct]; 7 (43 ): 69256-69266 PMID27626491 show ga To examine if brain neurons involved in the efferent control of the kidneys possess melanocortin-4 receptor (MC4-R) and/or tryptophan hydroxylase (TPH). Retrograde tracing pseudorabies virus (PRV)-614 was injected into the kidneys in adult male MC4R-green fluorescent protein (GFP) transgenic mice. After a survival time of 3-7 days, spinal cord and brain were removed and sectioned, and processed for PRV-614 visualization. The neurochemical phenotype of PRV-614-positive neurons was identified using double or triple immunocytochemical labeling against PRV-614, MC4R, or TPH. Double and triple labeling was quantified using microscopy. The majority of PRV-614 immunopositive neurons which also expressed immunoreactivity for MC4R were located in the ipsilateral intermediolateral cell column (IML) of the thoracic spinal cord, the paraventricular nucleus (PVN) of the hypothalamus, and raphe pallidus (RPa), nucleus raphe magnus (NRM) and ventromedial medulla (VMM) of the brainstem. Triple-labeled MC4R/PRV-614/TPH neurons were concentrated in the PVN, RPa, NRM and VMM. These data strongly suggest that central MC4R and TPH are involved in the efferent neuronal control of the kidneys. |Animals [MESH] |Brain/cytology/metabolism/virology [MESH] |Green Fluorescent Proteins/genetics/metabolism [MESH] |Herpesvirus 1, Suid/physiology [MESH] |Host-Pathogen Interactions [MESH] |Immunohistochemistry/methods [MESH] |Kidney/innervation/*metabolism/virology [MESH] |Male [MESH] |Mice, Transgenic [MESH] |Neural Pathways/*metabolism/virology [MESH] |Neurons/metabolism [MESH] |Receptor, Melanocortin, Type 4/genetics/*metabolism [MESH] |Spinal Cord/metabolism/virology [MESH]Delineation of the central melanocortin circuitry controlling the kidn(epmc).pdf DeepDyve Pubget Overpricing