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2016 ; 7
(44
): 71915-71921
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Automated analysis of acute myeloid leukemia minimal residual disease using a
support vector machine
#MMPMID27713120
Ni W
; Hu B
; Zheng C
; Tong Y
; Wang L
; Li QQ
; Tong X
; Han Y
Oncotarget
2016[Nov]; 7
(44
): 71915-71921
PMID27713120
show ga
We investigated the ability of support vector machines (SVM) to analyze minimal
residual disease (MRD) in flow cytometry data from patients with acute myeloid
leukemia (AML) automatically, objectively and standardly. The initial disease
data and MRD review data in the form of 159 flow cytometry standard 3.0 files
from 36 CD7-positive AML patients in whom MRD was detected more than once were
exported. SVM was used for training with setting the initial disease data to 1 as
the flag and setting 15 healthy persons to set 0 as the flag. Based on the two
training groups, parameters were optimized, and a predictive model was built to
analyze MRD data from each patient. The automated analysis results from the SVM
model were compared to those obtained through conventional analysis to determine
reliability. Automated analysis results based on the model did not differ from
and were correlated with results obtained through conventional analysis
(correlation coefficient c = 0.986, P > 0.05). Thus the SVM model could
potentially be used to analyze flow cytometry-based AML MRD data automatically,
objectively, and in a standardized manner.