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X-radiation enhances the collagen type I strap formation and migration potentials
of colon cancer cells
#MMPMID27655687
Blockhuys S
; Liu N
; Agarwal NR
; Enejder A
; Loitto V
; Sun XF
Oncotarget
2016[Nov]; 7
(44
): 71390-71399
PMID27655687
show ga
Rectal cancer treatment still fails with local and distant relapses of the
disease. It is hypothesized that radiotherapy could stimulate cancer cell
dissemination and metastasis. In this study, we evaluated the effect of
X-radiation on collagen type I strap formation potential, i.e. matrix remodeling
associated with mesenchymal cell migration, and behaviors of SW480, SW620, HCT116
p53+/+ and HCT116 p53-/- colon cancer cells. We determined a radiation-induced
increase in collagen type I strap formation and migration potentials of SW480 and
HCT116 p53+/+. Further studies with HCT116 p53+/+, indicated that after
X-radiation strap forming cells have an increased motility. More, we detected a
decrease in adhesion potential and mature integrin ?1 expression, but no change
in non-muscle myosin II expression for HCT116 p53+/+ after X-radiation. Integrin
?1 neutralization resulted in a decreased cell adhesion and collagen type I strap
formation in both sham and X-radiated conditions. Our study indicates collagen
type I strap formation as a potential mechanism of colon cancer cells with
increased migration potential after X-radiation, and suggests that other
molecules than integrin ?1 and non-muscle myosin II are responsible for the
radiation-induced collagen type I strap formation potential of colon cancer
cells. This work encourages further molecular investigation of radiation-induced
migration to improve rectal cancer treatment outcome.
|Cardiac Myosins/analysis
[MESH]
|Cell Adhesion/radiation effects
[MESH]
|Cell Line, Tumor
[MESH]
|Cell Movement/radiation effects
[MESH]
|Collagen Type I/*chemistry
[MESH]
|Colonic Neoplasms/*pathology
[MESH]
|Humans
[MESH]
|Integrin beta1/physiology
[MESH]
|Molecular Motor Proteins/analysis/physiology
[MESH]
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