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Correlation between positron emission tomography and Cerenkov luminescence
imaging in vivo and ex vivo using 64Cu-labeled antibodies in a neuroblastoma
mouse model
#MMPMID27602580
Maier FC
; Schmitt J
; Maurer A
; Ehrlichmann W
; Reischl G
; Nikolaou K
; Handgretinger R
; Pichler BJ
; Thaiss WM
Oncotarget
2016[Oct]; 7
(41
): 67403-67411
PMID27602580
show ga
Antibody-based therapies gain momentum in clinical therapy, thus the need for
accurate imaging modalities with respect to target identification and therapy
monitoring are of increasing relevance. Cerenkov luminescence imaging (CLI) are a
novel method detecting charged particles emitted during radioactive decay with
optical imaging. Here, we compare Position Emission Tomography (PET) with CLI in
a multimodal imaging study aiming at the fast and efficient screening of
monoclonal antibodies (mAb) designated for targeting of the
neuroblastoma-characteristic epitope disialoganglioside GD2.
Neuroblastoma-bearing SHO mice were injected with a 64Cu-labeled GD2-specific
mAb. The tumor uptake was imaged 3 h, 24 h and 48 h after tracer injection with
both, PET and CLI, and was compared to the accumulation in GD2-negative control
tumors (human embryonic kidney, HEK-293). In addition to an in vivo
PET/CLI-correlation over time, we also demonstrate linear correlations of CLI-
and ?-counter-based biodistribution analysis. CLI with its comparably short
acquisition time can thus be used as an attractive one-stop-shop modality for the
longitudinal monitoring of antibody-based tumor targeting and ex vivo
biodistribution.These findings suggest CLI as a reliable alternative for PET and
biodistribution studies with respect to fast and high-throughput screenings in
subcutaneous tumors traced with radiolabeled antibodies. However, in contrast to
PET, CLI is not limited to positron-emitting isotopes and can therefore also be
used for the visualization of mAb labeled with therapeutic isotopes like electron
emitters.
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