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10.18632/oncotarget.11888 http://scihub22266oqcxt.onion/10.18632/oncotarget.11888 C5341852!5341852!27611950     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Oncotarget 2016 ; 7 (41): 66989-7003 Nephropedia Template TP {{tp|p=27611950|t=2016. MicroRNA-21 promotes TGF-?1-induced epithelial-mesenchymal transition in gastric cancer through up-regulating PTEN expression |pdf=|usr=}}{{27611950}} gab.com Text MicroRNA-21 promotes TGF- 1-induced epithelial-mesenchymal transition in gastric cancer through up-regulating PTEN expression JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27611950 #FOAvip This study aimed to explore the effects of miR-21 and PTEN/Akt signaling pathway on TGF-?1-induced epithelial-mesenchymal transition (EMT) in gastric cancer (GC). GC tissues and adjacent tissues were collected from 83 patients. The qRT-PCR assay was performed to detect miR-21 expression. The expressions of PTEN, Akt and p-Akt were detected by immunohistochemistry. After 48 h of treatment with TGF-?1 (10 ng/mL), the SGC-7901 and KATO-III cells were divided into the blank, negative control (NC), miR-21 inhibitors, PTEN-siRNA and miR-21 inhibitors + PTEN-siRNA groups. EMT related factors and PTEN expressions were detected by qRT-PCR assay and Western blotting. The scratch test was conducted to observe cell migration. Xenograft tumor model in nude mice was used to evaluate the effects of miR-21 on EMT of GC cells in vivo. In GC tissues, the expressions of miR-21, Akt and p-Akt were up-regulated, while PTEN expression was down-regulated. Gene and protein expressions of E-cadherin and PTEN in the miR-21 inhibitors group were higher than the blank, NC, PTEN-siRNA and miR-21 inhibitors + PTEN-siRNA groups, while the expressions of N-cadherin, ?-catenin, Vimentin and Slug in the miR-21 inhibitors group were lower than other groups. MiR-21 inhibitors significantly inhibit cell migration and invasion in GC cell lines. In vivo xenograft experiment revealed that miR-21 inhibitor inhibits the growth of transplanted tumor through up-regulating E-cadherin and PTEN expressions and down-regulating the expressions of N-cadherin, ?-catenin, Vimentin and Slug. These results suggest that miR-21 could promote TGF-?1-induced EMT in GC cells through up-regulating PTEN expression. Twit Text FOAVip MicroRNA-21 promotes TGF- 1-induced epithelial-mesenchymal transition in gastric cancer through up-regulating PTEN expression ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27611950 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27611950 #FOAvip English Wikipedia <ref>{{Cite pmid|27611950}}</ref> MicroRNA-21 promotes TGF-?1-induced epithelial-mesenchymal transition in gastric cancer through up-regulating PTEN expression #MMPMID27611950 Li C; Song L; Zhang Z; Bai XX; Cui MF; Ma LJ Oncotarget 2016[Oct]; 7 (41): 66989-7003 PMID27611950show ga This study aimed to explore the effects of miR-21 and PTEN/Akt signaling pathway on TGF-?1-induced epithelial-mesenchymal transition (EMT) in gastric cancer (GC). GC tissues and adjacent tissues were collected from 83 patients. The qRT-PCR assay was performed to detect miR-21 expression. The expressions of PTEN, Akt and p-Akt were detected by immunohistochemistry. After 48 h of treatment with TGF-?1 (10 ng/mL), the SGC-7901 and KATO-III cells were divided into the blank, negative control (NC), miR-21 inhibitors, PTEN-siRNA and miR-21 inhibitors + PTEN-siRNA groups. EMT related factors and PTEN expressions were detected by qRT-PCR assay and Western blotting. The scratch test was conducted to observe cell migration. Xenograft tumor model in nude mice was used to evaluate the effects of miR-21 on EMT of GC cells in vivo. In GC tissues, the expressions of miR-21, Akt and p-Akt were up-regulated, while PTEN expression was down-regulated. Gene and protein expressions of E-cadherin and PTEN in the miR-21 inhibitors group were higher than the blank, NC, PTEN-siRNA and miR-21 inhibitors + PTEN-siRNA groups, while the expressions of N-cadherin, ?-catenin, Vimentin and Slug in the miR-21 inhibitors group were lower than other groups. MiR-21 inhibitors significantly inhibit cell migration and invasion in GC cell lines. In vivo xenograft experiment revealed that miR-21 inhibitor inhibits the growth of transplanted tumor through up-regulating E-cadherin and PTEN expressions and down-regulating the expressions of N-cadherin, ?-catenin, Vimentin and Slug. These results suggest that miR-21 could promote TGF-?1-induced EMT in GC cells through up-regulating PTEN expression. äMicroRNA-21 promotes TGF-?1-induced epithelial-mesenchymal transition (epmc).pdf DeepDyve Pubget Overpricing