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10.1091/mbc.E16-08-0612 http://scihub22266oqcxt.onion/10.1091/mbc.E16-08-0612 C5341730!5341730 !27903772     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27903772 &cmd=llinks): Failed to open stream: HTTP request failed! 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Diverse activities of viral cis-acting RNA regulatory elements revealed using multicolor, long-term, single-cell imaging |pdf=|usr=}}{{27903772 }} gab.com Text Diverse activities of viral cis-acting RNA regulatory elements revealed using multicolor, long-term, single-cell imaging JO: Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27903772 #FOAvip Cis-acting RNA structural elements govern crucial aspects of viral gene expression. How these structures and other posttranscriptional signals affect RNA trafficking and translation in the context of single cells is poorly understood. Herein we describe a multicolor, long-term (>24 h) imaging strategy for measuring integrated aspects of viral RNA regulatory control in individual cells. We apply this strategy to demonstrate differential mRNA trafficking behaviors governed by RNA elements derived from three retroviruses (HIV-1, murine leukemia virus, and Mason-Pfizer monkey virus), two hepadnaviruses (hepatitis B virus and woodchuck hepatitis virus), and an intron-retaining transcript encoded by the cellular NXF1 gene. Striking behaviors include "burst" RNA nuclear export dynamics regulated by HIV-1's Rev response element and the viral Rev protein; transient aggregations of RNAs into discrete foci at or near the nuclear membrane triggered by multiple elements; and a novel, pulsiform RNA export activity regulated by the hepadnaviral posttranscriptional regulatory element. We incorporate single-cell tracking and a data-mining algorithm into our approach to obtain RNA element-specific, high-resolution gene expression signatures. Together these imaging assays constitute a tractable, systems-based platform for studying otherwise difficult to access spatiotemporal features of viral and cellular gene regulation. Twit Text FOAVip Diverse activities of viral cis-acting RNA regulatory elements revealed using multicolor, long-term, single-cell imaging ????Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27903772 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27903772 #FOAvip English Wikipedia <ref>{{Cite pmid|27903772 }}</ref> Diverse activities of viral cis-acting RNA regulatory elements revealed using multicolor, long-term, single-cell imaging #MMPMID27903772 Pocock GM ; Zimdars LL ; Yuan M ; Eliceiri KW ; Ahlquist P ; Sherer NM Mol Biol Cell 2017[Feb]; 28 (3 ): 476-487 PMID27903772 show ga Cis-acting RNA structural elements govern crucial aspects of viral gene expression. How these structures and other posttranscriptional signals affect RNA trafficking and translation in the context of single cells is poorly understood. Herein we describe a multicolor, long-term (>24 h) imaging strategy for measuring integrated aspects of viral RNA regulatory control in individual cells. We apply this strategy to demonstrate differential mRNA trafficking behaviors governed by RNA elements derived from three retroviruses (HIV-1, murine leukemia virus, and Mason-Pfizer monkey virus), two hepadnaviruses (hepatitis B virus and woodchuck hepatitis virus), and an intron-retaining transcript encoded by the cellular NXF1 gene. Striking behaviors include "burst" RNA nuclear export dynamics regulated by HIV-1's Rev response element and the viral Rev protein; transient aggregations of RNAs into discrete foci at or near the nuclear membrane triggered by multiple elements; and a novel, pulsiform RNA export activity regulated by the hepadnaviral posttranscriptional regulatory element. We incorporate single-cell tracking and a data-mining algorithm into our approach to obtain RNA element-specific, high-resolution gene expression signatures. Together these imaging assays constitute a tractable, systems-based platform for studying otherwise difficult to access spatiotemporal features of viral and cellular gene regulation. |Active Transport, Cell Nucleus/physiology [MESH] |Cell Nucleus/metabolism [MESH] |Gene Expression Regulation, Viral [MESH] |Gene Products, rev/metabolism [MESH] |Genes, env/physiology [MESH] |HIV-1 [MESH] |Mason-Pfizer monkey virus [MESH] |Molecular Imaging/*methods [MESH] |RNA Processing, Post-Transcriptional/physiology [MESH] |RNA, Messenger/metabolism [MESH] |RNA, Viral [MESH] |Regulatory Sequences, Nucleic Acid/genetics/physiology [MESH] |Regulatory Sequences, Ribonucleic Acid/genetics/physiology [MESH]Diverse activities of viral cis-acting RNA regulatory elements reveale(epmc).pdf DeepDyve Pubget Overpricing