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10.1128/mBio.01977-16 http://scihub22266oqcxt.onion/10.1128/mBio.01977-16 C5340871!5340871!28270582     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       mBio 2017 ; 8 (2): ä Nephropedia Template TP {{tp|p=28270582|t=2017. Bacteria Getting into Shape: Genetic Determinants of E  coli Morphology |pdf=|usr=}}{{28270582}} gab.com Text Bacteria Getting into Shape: Genetic Determinants of E  coli Morphology JO: mBio http://www.kidney.de/pget.php?&tw=1&pmid=28270582 #FOAvip Perturbation of cellular processes is a prevailing approach to understanding biology. To better understand the complicated biology that defines bacterial shape, a sensitive, high-content platform was developed to detect multiple morphological defect phenotypes using microscopy. We examined morphological phenotypes across the Escherichia coli K-12 deletion (Keio) collection at the mid-exponential growth phase, revealing 111 deletions perturbing shape. Interestingly, 64% of these were uncharacterized mutants, illustrating the complex nature of shape maintenance and regulation in bacteria. To understand the roles these genes play in defining morphology, 53 mutants with knockouts resulting in abnormal cell shape were crossed with the Keio collection in high throughput, generating 1,373 synthetic lethal interactions across 1.7 million double deletion mutants. This analysis yielded a highly populated interaction network spanning and linking multiple phenotypes, with a preponderance of interactions involved in transport, oxidation-reduction, and metabolic processes. Twit Text FOAVip Bacteria Getting into Shape: Genetic Determinants of E  coli Morphology ????mBio http://www.kidney.de/pget.php?&tw=1&pmid=28270582 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28270582 #FOAvip English Wikipedia <ref>{{Cite pmid|28270582}}</ref> Bacteria Getting into Shape: Genetic Determinants of E  coli Morphology #MMPMID28270582 French S; Côté JP; Stokes JM; Truant R; Brown ED mBio 2017[Mar]; 8 (2): ä PMID28270582show ga Perturbation of cellular processes is a prevailing approach to understanding biology. To better understand the complicated biology that defines bacterial shape, a sensitive, high-content platform was developed to detect multiple morphological defect phenotypes using microscopy. We examined morphological phenotypes across the Escherichia coli K-12 deletion (Keio) collection at the mid-exponential growth phase, revealing 111 deletions perturbing shape. Interestingly, 64% of these were uncharacterized mutants, illustrating the complex nature of shape maintenance and regulation in bacteria. To understand the roles these genes play in defining morphology, 53 mutants with knockouts resulting in abnormal cell shape were crossed with the Keio collection in high throughput, generating 1,373 synthetic lethal interactions across 1.7 million double deletion mutants. This analysis yielded a highly populated interaction network spanning and linking multiple phenotypes, with a preponderance of interactions involved in transport, oxidation-reduction, and metabolic processes. äBacteria Getting into Shape: Genetic Determinants of E  coli Morpholog(epmc).pdf DeepDyve Pubget Overpricing