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10.1074/jbc.M116.756197 http://scihub22266oqcxt.onion/10.1074/jbc.M116.756197 C5339771!5339771 !28082679     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28082679 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2017 ; 292 (9 ): 3909-3918 Nephropedia Template TP {{tp|p=28082679 |t=2017. Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells |pdf=|usr=}}{{28082679 }} gab.com Text Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28082679 #FOAvip Mast cells are secretory cells that play an important role in host defense by discharging various intragranular contents, such as histamine and serotonin, upon stimulation of Fc receptors. The granules also contain spermine and spermidine, which can act as modulators of mast cell function, although the mechanism underlying vesicular storage remains unknown. Vesicular polyamine transporter (VPAT), the fourth member of the SLC18 transporter family, is an active transporter responsible for vesicular storage of spermine and spermidine in neurons. In the present study, we investigated whether VPAT functions in mast cells. RT-PCR and Western blotting indicated VPAT expression in murine bone marrow-derived mast cells (BMMCs). Immunohistochemical analysis indicated that VPAT is colocalized with VAMP3 but not with histamine, serotonin, cathepsin D, VAMP2, or VAMP7. Membrane vesicles from BMMCs accumulated spermidine upon the addition of ATP in a reserpine- and bafilomycin A(1)-sensitive manner. BMMCs secreted spermine and spermidine upon the addition of either antigen or A23187 in the presence of Ca(2+), and the antigen-mediated release, which was shown to be temperature-dependent and sensitive to bafilomycin A(1) and tetanus toxin, was significantly suppressed by VPAT gene RNA interference. Under these conditions, expression of vesicular monoamine transporter 2 was unaffected, but antigen-dependent histamine release was significantly suppressed, which was recovered by the addition of 1 mm spermine. These results strongly suggest that VPAT is expressed and is responsible for vesicular storage of spermine and spermidine in novel secretory granules that differ from histamine- and serotonin-containing granules and is involved in vesicular release of these polyamines from mast cells. Twit Text FOAVip Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28082679 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28082679 #FOAvip English Wikipedia <ref>{{Cite pmid|28082679 }}</ref> Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells #MMPMID28082679 Takeuchi T ; Harada Y ; Moriyama S ; Furuta K ; Tanaka S ; Miyaji T ; Omote H ; Moriyama Y ; Hiasa M J Biol Chem 2017[Mar]; 292 (9 ): 3909-3918 PMID28082679 show ga Mast cells are secretory cells that play an important role in host defense by discharging various intragranular contents, such as histamine and serotonin, upon stimulation of Fc receptors. The granules also contain spermine and spermidine, which can act as modulators of mast cell function, although the mechanism underlying vesicular storage remains unknown. Vesicular polyamine transporter (VPAT), the fourth member of the SLC18 transporter family, is an active transporter responsible for vesicular storage of spermine and spermidine in neurons. In the present study, we investigated whether VPAT functions in mast cells. RT-PCR and Western blotting indicated VPAT expression in murine bone marrow-derived mast cells (BMMCs). Immunohistochemical analysis indicated that VPAT is colocalized with VAMP3 but not with histamine, serotonin, cathepsin D, VAMP2, or VAMP7. Membrane vesicles from BMMCs accumulated spermidine upon the addition of ATP in a reserpine- and bafilomycin A(1)-sensitive manner. BMMCs secreted spermine and spermidine upon the addition of either antigen or A23187 in the presence of Ca(2+), and the antigen-mediated release, which was shown to be temperature-dependent and sensitive to bafilomycin A(1) and tetanus toxin, was significantly suppressed by VPAT gene RNA interference. Under these conditions, expression of vesicular monoamine transporter 2 was unaffected, but antigen-dependent histamine release was significantly suppressed, which was recovered by the addition of 1 mm spermine. These results strongly suggest that VPAT is expressed and is responsible for vesicular storage of spermine and spermidine in novel secretory granules that differ from histamine- and serotonin-containing granules and is involved in vesicular release of these polyamines from mast cells. |Animals [MESH] |Calcimycin/chemistry [MESH] |Calcium/chemistry [MESH] |Cathepsin D/chemistry [MESH] |Cation Transport Proteins/*metabolism [MESH] |Exocytosis [MESH] |Histamine Release [MESH] |Histamine/chemistry [MESH] |Immunohistochemistry [MESH] |Male [MESH] |Mast Cells/cytology/*metabolism [MESH] |Mice [MESH] |Microscopy, Fluorescence [MESH] |Polyamines/*metabolism [MESH] |R-SNARE Proteins/metabolism [MESH] |Rats [MESH] |Rats, Wistar [MESH] |Secretory Vesicles/metabolism [MESH] |Serotonin/chemistry [MESH] |Spermidine/metabolism [MESH] |Spermine/metabolism [MESH] |Vesicle-Associated Membrane Protein 2/metabolism [MESH] |Vesicle-Associated Membrane Protein 3/metabolism [MESH]Vesicular Polyamine Transporter Mediates Vesicular Storage and Release(epmc).pdf DeepDyve Pubget Overpricing