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2017 ; 24
(3
): ä Nephropedia Template TP
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Otitis-Prone Children Produce Functional Antibodies to Pneumolysin and
Pneumococcal Polysaccharides
#MMPMID28031178
Kirkham LS
; Wiertsema SP
; Corscadden KJ
; Mateus T
; Mullaney GL
; Zhang G
; Richmond PC
; Thornton RB
Clin Vaccine Immunol
2017[Mar]; 24
(3
): ä PMID28031178
show ga
The pneumococcus is a major otitis media (OM) pathogen, but data are conflicting
regarding whether otitis-prone children have impaired humoral immunity to
pneumococcal antigens. We and others have shown that otitis-prone and healthy
children have similar antibody titers to pneumococcal proteins and
polysaccharides (vaccine and nonvaccine types); however, the quality of
antibodies from otitis-prone children has not been investigated. Antibody
function, rather than titer, is considered to be a better correlate of protection
from pneumococcal disease. Therefore, we compared the capacities of antibodies
from otitis-prone (cases) and healthy (controls) children to neutralize
pneumolysin, the pneumococcal toxin currently in development as a vaccine
antigen, and to opsonize pneumococcal vaccine and nonvaccine serotypes. A
pneumolysin neutralization assay was conducted on cholesterol-depleted
complement-inactivated sera from 165 cases and 61 controls. A multiplex
opsonophagocytosis assay (MOPA) was conducted on sera from 20 cases and 20
controls. Neutralizing and opsonizing titers were calculated with
antigen-specific IgG titers to determine antibody potency for pneumolysin,
pneumococcal conjugate vaccine (PCV) polysaccharides, and non-PCV
polysaccharides. There was no significant difference in antibody potencies
between cases and controls for the antigens tested. Antipneumolysin neutralizing
titers increased with the number of episodes of acute OM, but antibody potency
did not. Pneumolysin antibody potency was lower in children colonized with
pneumococci than in noncarriers, and this was significant for the otitis-prone
group (P < 0.05). The production of functional antipneumococcal antibodies in
otitis-prone children demonstrates that they respond to the current PCV and are
likely to respond to pneumolysin-based vaccines as effectively as healthy
children.
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