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2017 ; 2017
(ä): 7309481
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Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells
Anchored to Magnetic Nanoparticles
#MMPMID28316987
Liu Y
; Wang Y
; Yan M
; Huang J
Biomed Res Int
2017[]; 2017
(ä): 7309481
PMID28316987
show ga
Hierarchical copper shells anchored on magnetic nanoparticles were designed and
fabricated to selectively deplete hemoglobin from human blood by immobilized
metal affinity chromatography. Briefly, CoFe(2)O(4) nanoparticles coated with
polyacrylic acid were first synthesized by a one-pot solvothermal method.
Hierarchical copper shells were then deposited by immobilizing Cu(2+) on
nanoparticles and subsequently by reducing between the solid CoFe(2)O(4)@COOH and
copper solution with NaBH(4). The resulting nanoparticles were characterized by
scanning electron microscopy, transmission electron microscopy, Fourier transform
infrared spectrometry, X-ray photoelectron spectroscopy, and vibrating sample
magnetometry. The particles were also tested against purified bovine hemoglobin
over a range of pH, contact time, and initial protein concentration. Hemoglobin
adsorption followed pseudo-second-order kinetics and reached equilibrium in
90?min. Isothermal data also fit the Langmuir model well, with calculated maximum
adsorption capacity 666?mg g(-1). Due to the high density of Cu(2+) on the shell,
the nanoparticles efficiently and selectively deplete hemoglobin from human
blood. Taken together, the results demonstrate that the particles with
hierarchical copper shells effectively remove abundant, histidine-rich proteins,
such as hemoglobin from human blood, and thereby minimize interference in
diagnostic and other assays.