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Strain-specific probiotic properties of lactic acid bacteria and their
interference with human intestinal pathogens invasion
#MMPMID28286570
Campana R
; van Hemert S
; Baffone W
Gut Pathog
2017[]; 9
(?): 12
PMID28286570
show ga
BACKGROUND: One of the working mechanisms of probiotic bacteria is their ability
to compete with pathogens. To define the probiotic properties of seven Lactic
Acid Bacteria (LAB) strains, we tested them for survival in simulated
gastro-intestinal conditions, antimicrobial activities, co-aggregative abilities,
and interferences studies against five human intestinal pathogens (Salmonella
enteritidis ATCC 13076, Listeria monocytogenes ATCC 7644, Escherichia coli O157:
H7 ATCC 35150, Cronobacter sakazakii ATCC 29544 and Campylobacter jejuni ATCC
33291). RESULTS: The LAB strains were able to survive the stomach simulated
conditions, and varied in their abilities to survive the small
intestinal-simulated conditions. The strains showed antibiotic susceptibility
profiles with values equal or below the breakpoints set by the European Food and
Safety Authority. The LAB cell-free cultures supernatants showed antimicrobial
activities, with inhibition zones ranging from 10.0 to 17.2 mm. All the LAB
strains showed moderate auto-aggregation abilities while the greatest
co-aggregation abilities were observed for Bifidobacterium bifidum W23,
Lactobacillus plantarum W21 and Lactobacillus rhamnosus W71. The individual LAB
strains showed strain-specific abilities to reduce the invasion of intestinal
pathogens in an interference model with Caco-2 cells. Increased invasion
inhibition was found when different combinations of LAB strains were used in the
interference tests. CONCLUSION: The LAB strains examined in this study may
protect the intestinal epithelium through a series of barriers (antimicrobial
activity, co-aggregation with pathogens, adherence) and interference mechanisms.
Consequently, these LAB strains may be considered candidates for prophylactic use
to prevent intestinal infections.