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10.1186/s13059-017-1171-9 http://scihub22266oqcxt.onion/10.1186/s13059-017-1171-9 C5333448!5333448!28249587     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Biol 2017 ; 18 (ä): ä Nephropedia Template TP {{tp|p=28249587|t=2017. Single-cell transcriptome conservation in cryopreserved cells and tissues |pdf=|usr=}}{{28249587}} gab.com Text Single-cell transcriptome conservation in cryopreserved cells and tissues JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28249587 #FOAvip A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1171-9) contains supplementary material, which is available to authorized users. Twit Text FOAVip Single-cell transcriptome conservation in cryopreserved cells and tissues ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=28249587 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28249587 #FOAvip English Wikipedia <ref>{{Cite pmid|28249587}}</ref> Single-cell transcriptome conservation in cryopreserved cells and tissues #MMPMID28249587 Guillaumet-Adkins A; Rodríguez-Esteban G; Mereu E; Mendez-Lago M; Jaitin DA; Villanueva A; Vidal A; Martinez-Marti A; Felip E; Vivancos A; Keren-Shaul H; Heath S; Gut M; Amit I; Gut I; Heyn H Genome Biol 2017[]; 18 (ä): ä PMID28249587show ga A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-017-1171-9) contains supplementary material, which is available to authorized users. äSingle-cell transcriptome conservation in cryopreserved cells and tiss(epmc).pdf DeepDyve Pubget Overpricing