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10.3390/genes8020062 http://scihub22266oqcxt.onion/10.3390/genes8020062 C5333051!5333051!28208743     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28208743&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Genes+(Basel) 2017 ; 8 (2): ä Nephropedia Template TP {{tp|p=28208743|t=2017. Elaborated Action of the Human Primosome |pdf=|usr=}}{{28208743}} gab.com Text Elaborated Action of the Human Primosome JO: Genes (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=28208743 #FOAvip The human primosome is a 340-kilodalton complex of primase (DNA-dependent RNA polymerase) and DNA polymerase ?, which initiates genome replication by synthesizing chimeric RNA-DNA primers for DNA polymerases ? and ?. Accumulated biochemical and structural data reveal the complex mechanism of concerted primer synthesis by two catalytic centers. First, primase generates an RNA primer through three steps: initiation, consisting of dinucleotide synthesis from two nucleotide triphosphates; elongation, resulting in dinucleotide extension; and termination, owing to primase inhibition by a mature 9-mer primer. Then Pol?, which works equally well on DNA:RNA and DNA:DNA double helices, intramolecularly catches the template primed by a 9-mer RNA and extends the primer with dNTPs. All primosome transactions are highly coordinated by autoregulation through the alternating activation/inhibition of the catalytic centers. This coordination is mediated by the small C-terminal domain of the primase accessory subunit, which forms a tight complex with the template:primer, shuttles between the primase and DNA polymerase active sites, and determines their access to the substrate. Twit Text FOAVip Elaborated Action of the Human Primosome ????Genes (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=28208743 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28208743 #FOAvip English Wikipedia <ref>{{Cite pmid|28208743}}</ref> Elaborated Action of the Human Primosome #MMPMID28208743 Baranovskiy AG; Tahirov TH Genes (Basel) 2017[Feb]; 8 (2): ä PMID28208743show ga The human primosome is a 340-kilodalton complex of primase (DNA-dependent RNA polymerase) and DNA polymerase ?, which initiates genome replication by synthesizing chimeric RNA-DNA primers for DNA polymerases ? and ?. Accumulated biochemical and structural data reveal the complex mechanism of concerted primer synthesis by two catalytic centers. First, primase generates an RNA primer through three steps: initiation, consisting of dinucleotide synthesis from two nucleotide triphosphates; elongation, resulting in dinucleotide extension; and termination, owing to primase inhibition by a mature 9-mer primer. Then Pol?, which works equally well on DNA:RNA and DNA:DNA double helices, intramolecularly catches the template primed by a 9-mer RNA and extends the primer with dNTPs. All primosome transactions are highly coordinated by autoregulation through the alternating activation/inhibition of the catalytic centers. This coordination is mediated by the small C-terminal domain of the primase accessory subunit, which forms a tight complex with the template:primer, shuttles between the primase and DNA polymerase active sites, and determines their access to the substrate. äElaborated Action of the Human Primosome (epmc).pdf DeepDyve Pubget Overpricing