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10.1038/sdata.2017.8

http://scihub22266oqcxt.onion/10.1038/sdata.2017.8
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C5332015!5332015!28248925
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suck abstract from ncbi


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pmid28248925      Sci+Data 2017 ; 4 (ä): ä
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  • Genome-wide siRNA screen of genes regulating the LPS-induced NF-?B and TNF-? responses in mouse macrophages #MMPMID28248925
  • Li N; Katz S; Dutta B; Benet ZL; Sun J; Fraser ID
  • Sci Data 2017[]; 4 (ä): ä PMID28248925show ga
  • The mammalian innate immune system senses many bacterial stimuli through the toll-like receptor (TLR) family. Activation of the TLR4 receptor by bacterial lipopolysaccharide (LPS) is the most widely studied TLR pathway due to its central role in host responses to gram-negative bacterial infection and its contribution to endotoxemia and sepsis. Here we describe a genome-wide siRNA screen to identify genes regulating the mouse macrophage TNF-? and NF-?B responses to LPS. We include a secondary validation screen conducted with six independent siRNAs per gene to facilitate removal of off-target screen hits. We also provide microarray data from the same LPS-treated macrophage cells to facilitate downstream data analysis. These data provide a resource for analyzing gene function in the predominant pathway driving inflammatory signaling and cytokine expression in mouse macrophages.
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