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10.1038/sdata.2017.8 http://scihub22266oqcxt.onion/10.1038/sdata.2017.8 C5332015!5332015 !28248925     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28248925 &cmd=llinks): Failed to open stream: HTTP request failed! 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Genome-wide siRNA screen of genes regulating the LPS-induced NF-?B and TNF-? responses in mouse macrophages |pdf=|usr=}}{{28248925 }} gab.com Text Genome-wide siRNA screen of genes regulating the LPS-induced NF- B and TNF- responses in mouse macrophages JO: Sci Data http://www.kidney.de/pget.php?&tw=1&pmid=28248925 #FOAvip The mammalian innate immune system senses many bacterial stimuli through the toll-like receptor (TLR) family. Activation of the TLR4 receptor by bacterial lipopolysaccharide (LPS) is the most widely studied TLR pathway due to its central role in host responses to gram-negative bacterial infection and its contribution to endotoxemia and sepsis. Here we describe a genome-wide siRNA screen to identify genes regulating the mouse macrophage TNF-? and NF-?B responses to LPS. We include a secondary validation screen conducted with six independent siRNAs per gene to facilitate removal of off-target screen hits. We also provide microarray data from the same LPS-treated macrophage cells to facilitate downstream data analysis. These data provide a resource for analyzing gene function in the predominant pathway driving inflammatory signaling and cytokine expression in mouse macrophages. Twit Text FOAVip Genome-wide siRNA screen of genes regulating the LPS-induced NF- B and TNF- responses in mouse macrophages ????Sci Data http://www.kidney.de/pget.php?&tw=1&pmid=28248925 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28248925 #FOAvip English Wikipedia <ref>{{Cite pmid|28248925 }}</ref> Genome-wide siRNA screen of genes regulating the LPS-induced NF-?B and TNF-? responses in mouse macrophages #MMPMID28248925 Li N ; Katz S ; Dutta B ; Benet ZL ; Sun J ; Fraser ID Sci Data 2017[Mar]; 4 (ä): 170008 PMID28248925 show ga The mammalian innate immune system senses many bacterial stimuli through the toll-like receptor (TLR) family. Activation of the TLR4 receptor by bacterial lipopolysaccharide (LPS) is the most widely studied TLR pathway due to its central role in host responses to gram-negative bacterial infection and its contribution to endotoxemia and sepsis. Here we describe a genome-wide siRNA screen to identify genes regulating the mouse macrophage TNF-? and NF-?B responses to LPS. We include a secondary validation screen conducted with six independent siRNAs per gene to facilitate removal of off-target screen hits. We also provide microarray data from the same LPS-treated macrophage cells to facilitate downstream data analysis. These data provide a resource for analyzing gene function in the predominant pathway driving inflammatory signaling and cytokine expression in mouse macrophages. |Animals [MESH] |Gene Expression Regulation [MESH] |Humans [MESH] |Lipopolysaccharides [MESH] |Macrophage Activation/*genetics [MESH] |Macrophages/*metabolism [MESH] |NF-kappa B/genetics/*metabolism [MESH] |RNA, Small Interfering [MESH]Genome-wide siRNA screen of genes regulating the LPS-induced NF-?B and(epmc).pdf DeepDyve Pubget Overpricing