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10.1038/sdata.2017.22

http://scihub22266oqcxt.onion/10.1038/sdata.2017.22
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C5332014!5332014 !28248923
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suck abstract from ncbi

pmid28248923
      Sci+Data 2017 ; 4 (?): 170022
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  • Image-based siRNA screen to identify kinases regulating Weibel-Palade body size control using electroporation #MMPMID28248923
  • Ketteler R ; Freeman J ; Ferraro F ; Bata N ; Cutler DF ; Kriston-Vizi J ; Stevenson N
  • Sci Data 2017[Mar]; 4 (?): 170022 PMID28248923 show ga
  • High-content screening of kinase inhibitors is important in order to identify biogenesis and function mechanisms of subcellular organelles. Here, we present a human kinome siRNA high-content screen on primary human umbilical vein endothelial cells, that were transfected by electroporation. The data descriptor contains a confocal fluorescence, microscopic image dataset. We also describe an open source, automated image analysis workflow that can be reused to perform high-content analysis of other organelles. This dataset is suitable for analysis of morphological parameters that are linked to human umbilical vein endothelial cell (HUVEC) biology.
  • |*Weibel-Palade Bodies [MESH]
  • |Cell Line [MESH]
  • |Electroporation [MESH]
  • |Endothelium, Vascular [MESH]
  • |Humans [MESH]
  • |Protein Kinases [MESH]


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