Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/nature20565 http://scihub22266oqcxt.onion/10.1038/nature20565 C5331785!5331785!27851729     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nature 2016 ; 540 (7631): 144-9 Nephropedia Template TP {{tp|p=27851729|t=2016. In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration |pdf=|usr=}}{{27851729}} gab.com Text In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration JO: Nature http://www.kidney.de/pget.php?&tw=1&pmid=27851729 #FOAvip Targeted genome editing via engineered nucleases is an exciting area of biomedical research and holds potential for clinical applications. Despite rapid advances in the field, in vivo targeted transgene integration is still infeasible because current tools are inefficient1, especially for non-dividing cells, which compose most adult tissues. This poses a barrier for uncovering fundamental biological principles and developing treatments for a broad range of genetic disorders2. Based on clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9)3,4 technology, here we devise a homology-independent targeted integration (HITI) strategy, which allows for robust DNA knock-in in both dividing and non-dividing cells in vitro and, more importantly, in vivo (for example, in neurons of postnatal mammals). As a proof of concept of its therapeutic potential, we demonstrate the efficacy of HITI in improving visual function using a rat model of the retinal degeneration condition retinitis pigmentosa. The HITI method presented here establishes new avenues for basic research and targeted gene therapies. Twit Text FOAVip In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration ????Nature http://www.kidney.de/pget.php?&tw=1&pmid=27851729 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27851729 #FOAvip English Wikipedia <ref>{{Cite pmid|27851729}}</ref> In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration #MMPMID27851729 Suzuki K; Tsunekawa Y; Hernandez-Benitez R; Wu J; Zhu J; Kim EJ; Hatanaka F; Yamamoto M; Araoka T; Li Z; Kurita M; Hishida T; Li M; Aizawa E; Guo S; Chen S; Goebl A; Soligalla RD; Qu J; Jiang T; Fu X; Jafari M; Esteban CR; Berggren WT; Lajara J; Nuņez-Delicado E; Guillen P; Campistol JM; Matsuzaki F; Liu GH; Magistretti P; Zhang K; Callaway EM; Zhang K; Belmonte JCI Nature 2016[Dec]; 540 (7631): 144-9 PMID27851729show ga Targeted genome editing via engineered nucleases is an exciting area of biomedical research and holds potential for clinical applications. Despite rapid advances in the field, in vivo targeted transgene integration is still infeasible because current tools are inefficient1, especially for non-dividing cells, which compose most adult tissues. This poses a barrier for uncovering fundamental biological principles and developing treatments for a broad range of genetic disorders2. Based on clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9)3,4 technology, here we devise a homology-independent targeted integration (HITI) strategy, which allows for robust DNA knock-in in both dividing and non-dividing cells in vitro and, more importantly, in vivo (for example, in neurons of postnatal mammals). As a proof of concept of its therapeutic potential, we demonstrate the efficacy of HITI in improving visual function using a rat model of the retinal degeneration condition retinitis pigmentosa. The HITI method presented here establishes new avenues for basic research and targeted gene therapies. äIn vivo genome editing via CRISPR/Cas9 mediated homology-independent t(epmc).pdf DeepDyve Pubget Overpricing