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10.1364/BOE.8.001257

http://scihub22266oqcxt.onion/10.1364/BOE.8.001257
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C5330582!5330582!28271016
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suck abstract from ncbi


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pmid28271016      Biomed+Opt+Express 2017 ; 8 (2): 1257-70
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  • X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue #MMPMID28271016
  • Zdora MC; Vila-Comamala J; Schulz G; Khimchenko A; Hipp A; Cook AC; Dilg D; David C; Grünzweig C; Rau C; Thibault P; Zanette I
  • Biomed Opt Express 2017[Feb]; 8 (2): 1257-70 PMID28271016show ga
  • The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM.
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