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10.1080/20013078.2016.1272832 http://scihub22266oqcxt.onion/10.1080/20013078.2016.1272832 C5328325!5328325!28326168     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Extracell+Vesicles 2017 ; 6 (1): ä Nephropedia Template TP {{tp|p=28326168|t=2017. Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?|pdf=|usr=}}{{28326168}} gab.com Text Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components JO: J Extracell Vesicles http://www.kidney.de/pget.php?&tw=1&pmid=28326168 #FOAvip In a recently published study, Anna Krichevsky and colleagues raise the important question of whether results of in vitro extracellular RNA (exRNA) studies, including extracellular vesicle (EV) investigations, are confounded by the presence of RNA in cell culture medium components such as foetal bovine serum (FBS). The answer, according to their data, is a resounding ?yes?. Even after lengthy ultracentrifugation to remove bovine EVs from FBS, the majority of exRNA in FBS remained. Although technical factors may affect the degree of depletion, residual EVs and exRNA in FBS could influence the conclusions of in vitro studies: certainly, for secreted RNA, and possibly also for cell-associated RNA. In this commentary, we critically examine some of the literature in this field, including a recent study from some of the authors of this piece, in light of the Wei et al. study and explore how cell culture-derived RNAs may affect what we think we know about EV RNAs. These findings hold particular consequence as the field moves towards a deeper understanding of EV?RNA associations and potential functions. Twit Text FOAVip Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components ????J Extracell Vesicles http://www.kidney.de/pget.php?&tw=1&pmid=28326168 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28326168 #FOAvip English Wikipedia <ref>{{Cite pmid|28326168}}</ref> Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components? #MMPMID28326168 Tosar JP; Cayota A; Eitan E; Halushka MK; Witwer KW J Extracell Vesicles 2017[]; 6 (1): ä PMID28326168show ga In a recently published study, Anna Krichevsky and colleagues raise the important question of whether results of in vitro extracellular RNA (exRNA) studies, including extracellular vesicle (EV) investigations, are confounded by the presence of RNA in cell culture medium components such as foetal bovine serum (FBS). The answer, according to their data, is a resounding ?yes?. Even after lengthy ultracentrifugation to remove bovine EVs from FBS, the majority of exRNA in FBS remained. Although technical factors may affect the degree of depletion, residual EVs and exRNA in FBS could influence the conclusions of in vitro studies: certainly, for secreted RNA, and possibly also for cell-associated RNA. In this commentary, we critically examine some of the literature in this field, including a recent study from some of the authors of this piece, in light of the Wei et al. study and explore how cell culture-derived RNAs may affect what we think we know about EV RNAs. These findings hold particular consequence as the field moves towards a deeper understanding of EV?RNA associations and potential functions. äRibonucleic artefacts: are some extracellular RNA discoveries driven b(epmc).pdf DeepDyve Pubget Overpricing