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10.15252/msb.20167233 http://scihub22266oqcxt.onion/10.15252/msb.20167233 C5327727!5327727!28193641     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Syst+Biol 2017 ; 13 (2): ä Nephropedia Template TP {{tp|p=28193641|t=2017. A method for high?throughput production of sequence?verified DNA libraries and strain collections |pdf=|usr=}}{{28193641}} gab.com Text A method for high throughput production of sequence verified DNA libraries and strain collections JO: Mol Syst Biol http://www.kidney.de/pget.php?&tw=1&pmid=28193641 #FOAvip The low costs of array?synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error rates, uneven representation, and lack of access to individual oligonucleotides limit the true potential of these libraries. We have developed a cost?effective method called Recombinase Directed Indexing (REDI), which involves integration of a complex library into yeast, site?specific recombination to index library DNA, and next?generation sequencing to identify desired clones. We used REDI to generate a library of ~3,300 DNA probes that exhibited > 96% purity and remarkable uniformity (> 95% of probes within twofold of the median abundance). Additionally, we created a collection of ~9,000 individually accessible CRISPR interference yeast strains for > 99% of genes required for either fermentative or respiratory growth, demonstrating the utility of REDI for rapid and cost?effective creation of strain collections from oligonucleotide pools. Our approach is adaptable to any complex DNA library, and fundamentally changes how these libraries can be parsed, maintained, propagated, and characterized. Twit Text FOAVip A method for high throughput production of sequence verified DNA libraries and strain collections ????Mol Syst Biol http://www.kidney.de/pget.php?&tw=1&pmid=28193641 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28193641 #FOAvip English Wikipedia <ref>{{Cite pmid|28193641}}</ref> A method for high?throughput production of sequence?verified DNA libraries and strain collections #MMPMID28193641 Smith JD; Schlecht U; Xu W; Suresh S; Horecka J; Proctor MJ; Aiyar RS; Bennett RAO; Chu A; Li YF; Roy K; Davis RW; Steinmetz LM; Hyman RW; Levy SF; St.Onge RP Mol Syst Biol 2017[Feb]; 13 (2): ä PMID28193641show ga The low costs of array?synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error rates, uneven representation, and lack of access to individual oligonucleotides limit the true potential of these libraries. We have developed a cost?effective method called Recombinase Directed Indexing (REDI), which involves integration of a complex library into yeast, site?specific recombination to index library DNA, and next?generation sequencing to identify desired clones. We used REDI to generate a library of ~3,300 DNA probes that exhibited > 96% purity and remarkable uniformity (> 95% of probes within twofold of the median abundance). Additionally, we created a collection of ~9,000 individually accessible CRISPR interference yeast strains for > 99% of genes required for either fermentative or respiratory growth, demonstrating the utility of REDI for rapid and cost?effective creation of strain collections from oligonucleotide pools. Our approach is adaptable to any complex DNA library, and fundamentally changes how these libraries can be parsed, maintained, propagated, and characterized. äA method for high?throughput production of sequence?verified DNA libra(epmc).pdf DeepDyve Pubget Overpricing