Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1371/journal.pone.0173050 http://scihub22266oqcxt.onion/10.1371/journal.pone.0173050 C5325610!5325610 !28235049     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28235049 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2017 ; 12 (2 ): e0173050 Nephropedia Template TP {{tp|p=28235049 |t=2017. Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy |pdf=|usr=}}{{28235049 }} gab.com Text Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28235049 #FOAvip Continuous improvements in imaging techniques are challenging biologists to search for more accurate methods to label cellular elements. This is particularly relevant for diffraction-unlimited fluorescence imaging, where the perceived resolution is affected by the size of the affinity probes. This is evident when antibodies, which are 10-15 nm in size, are used. Previously it has been suggested that RNA aptamers (~3 nm) can be used to detect cellular proteins under super-resolution imaging. However, a direct comparison between several aptamers and antibodies is needed, to clearly show the advantages and/or disadvantages of the different probes. Here we have conducted such a comparative study, by testing several aptamers and antibodies using stimulated emission depletion microscopy (STED). We have targeted three membrane receptors, EGFR, ErbB2 and Epha2, which are relevant to human health, and recycle between plasma membrane and intracellular organelles. Our results suggest that the aptamers can reveal more epitopes than most antibodies, thus providing a denser labeling of the stained structures. Moreover, this improves the overall quality of the information that can be extracted from the images. We conclude that aptamers could become useful fluorescent labeling tools for light microscopy and super-resolution imaging, and that their development for novel targets is imperative. Twit Text FOAVip Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28235049 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28235049 #FOAvip English Wikipedia <ref>{{Cite pmid|28235049 }}</ref> Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy #MMPMID28235049 Gomes de Castro MA ; Höbartner C ; Opazo F PLoS One 2017[]; 12 (2 ): e0173050 PMID28235049 show ga Continuous improvements in imaging techniques are challenging biologists to search for more accurate methods to label cellular elements. This is particularly relevant for diffraction-unlimited fluorescence imaging, where the perceived resolution is affected by the size of the affinity probes. This is evident when antibodies, which are 10-15 nm in size, are used. Previously it has been suggested that RNA aptamers (~3 nm) can be used to detect cellular proteins under super-resolution imaging. However, a direct comparison between several aptamers and antibodies is needed, to clearly show the advantages and/or disadvantages of the different probes. Here we have conducted such a comparative study, by testing several aptamers and antibodies using stimulated emission depletion microscopy (STED). We have targeted three membrane receptors, EGFR, ErbB2 and Epha2, which are relevant to human health, and recycle between plasma membrane and intracellular organelles. Our results suggest that the aptamers can reveal more epitopes than most antibodies, thus providing a denser labeling of the stained structures. Moreover, this improves the overall quality of the information that can be extracted from the images. We conclude that aptamers could become useful fluorescent labeling tools for light microscopy and super-resolution imaging, and that their development for novel targets is imperative. |Antibodies/chemistry [MESH] |Antibody Affinity [MESH] |Aptamers, Nucleotide/*chemistry [MESH] |Endocytosis [MESH] |Epitopes/metabolism [MESH] |ErbB Receptors/*metabolism [MESH] |Fluorescent Antibody Technique, Indirect [MESH] |HEK293 Cells [MESH] |HeLa Cells [MESH] |Humans [MESH] |MCF-7 Cells [MESH] |Microscopy, Confocal [MESH] |Microscopy, Fluorescence [MESH] |Protein Binding [MESH] |Receptor, EphA2/*metabolism [MESH] |Receptor, ErbB-2/*metabolism [MESH]Aptamers provide superior stainings of cellular receptors studied unde(epmc).pdf DeepDyve Pubget Overpricing