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10.1111/apa.13683

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suck abstract from ncbi


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pmid27886403
      Acta+Paediatr 2017 ; 106 (3 ): 430-437
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  • Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity #MMPMID27886403
  • Verder H ; Heiring C ; Clark H ; Sweet D ; Jessen TE ; Ebbesen F ; Björklund LJ ; Andreasson B ; Bender L ; Bertelsen A ; Dahl M ; Eschen C ; Fenger-Grøn J ; Hoffmann SF ; Höskuldsson A ; Bruusgaard-Mouritsen M ; Lundberg F ; Postle AD ; Schousboe P ; Schmidt P ; Stanchev H ; Sørensen L
  • Acta Paediatr 2017[Mar]; 106 (3 ): 430-437 PMID27886403 show ga
  • AIM: Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation. METHODS: Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 ?L of aspirate. RESULTS: An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results. CONCLUSION: Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity.
  • |Bodily Secretions/chemistry [MESH]
  • |Female [MESH]
  • |Humans [MESH]
  • |Infant, Newborn [MESH]
  • |Lung/*growth & development [MESH]
  • |Male [MESH]
  • |Phosphatidylcholines/*analysis/metabolism [MESH]
  • |Respiratory Distress Syndrome, Newborn/*diagnosis [MESH]


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