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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Acta+Paediatr
2017 ; 106
(3
): 430-437
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Rapid test for lung maturity, based on spectroscopy of gastric aspirate,
predicted respiratory distress syndrome with high sensitivity
#MMPMID27886403
Verder H
; Heiring C
; Clark H
; Sweet D
; Jessen TE
; Ebbesen F
; Björklund LJ
; Andreasson B
; Bender L
; Bertelsen A
; Dahl M
; Eschen C
; Fenger-Grøn J
; Hoffmann SF
; Höskuldsson A
; Bruusgaard-Mouritsen M
; Lundberg F
; Postle AD
; Schousboe P
; Schmidt P
; Stanchev H
; Sørensen L
Acta Paediatr
2017[Mar]; 106
(3
): 430-437
PMID27886403
show ga
AIM: Respiratory distress syndrome (RDS) is a major cause of mortality and
morbidity in premature infants. By the time symptoms appear, it may already be
too late to prevent a severe course, with bronchopulmonary dysplasia or
mortality. We aimed to develop a rapid test of lung maturity for targeting
surfactant supplementation. METHODS: Concentrations of the most surface-active
lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric
aspirates from premature infants were measured by mass spectrometry and expressed
as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with
mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates
and oropharyngeal secretions from another group of premature infants using
spectroscopy and the results were compared with RDS development. The 10-minute
analysis required 10 ?L of aspirate. RESULTS: An L/S algorithm was developed
based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136
infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value
of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the
oropharyngeal secretions had less valid L/S than gastric aspirate results.
CONCLUSION: Our rapid test for lung maturity, based on spectroscopy of gastric
aspirate, predicted RDS with high sensitivity.