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10.1021/acscentsci.6b00330

http://scihub22266oqcxt.onion/10.1021/acscentsci.6b00330
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C5324081!5324081!28280778
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suck abstract from ncbi


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pmid28280778      ACS+Cent+Sci 2017 ; 3 (2): 117-23
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  • Two-Way Chemical Communication between Artificial and Natural Cells #MMPMID28280778
  • Lentini R; Martín NY; Forlin M; Belmonte L; Fontana J; Cornella M; Martini L; Tamburini S; Bentley WE; Jousson O; Mansy SS
  • ACS Cent Sci 2017[Feb]; 3 (2): 117-23 PMID28280778show ga
  • Artificial cells capable of both sensing and sending chemical messages to bacteria have yet to be built. Here we show that artificial cells that are able to sense and synthesize quorum signaling molecules can chemically communicate with V. fischeri, V. harveyi, E. coli, and P. aeruginosa. Activity was assessed by fluorescence, luminescence, RT-qPCR, and RNA-seq. Two potential applications for this technology were demonstrated. First, the extent to which artificial cells could imitate natural cells was quantified by a type of cellular Turing test. Artificial cells capable of sensing and in response synthesizing and releasing N-3-(oxohexanoyl)homoserine lactone showed a high degree of likeness to natural V. fischeri under specific test conditions. Second, artificial cells that sensed V. fischeri and in response degraded a quorum signaling molecule of P. aeruginosa (N-(3-oxododecanoyl)homoserine lactone) were constructed, laying the foundation for future technologies that control complex networks of natural cells.
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