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10.1038/srep43149 http://scihub22266oqcxt.onion/10.1038/srep43149 C5320493!5320493!28225089     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28225089|t=2017. The hidden perils of read mapping as a quality assessment tool in genome sequencing |pdf=|usr=}}{{28225089}} gab.com Text The hidden perils of read mapping as a quality assessment tool in genome sequencing JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28225089 #FOAvip This article provides a comparative analysis of the various methods of genome sequencing focusing on verification of the assembly quality. The results of a comparative assessment of various de novo assembly tools, as well as sequencing technologies, are presented using a recently completed sequence of the genome of Lactobacillus fermentum 3872. In particular, quality of assemblies is assessed by using CLC Genomics Workbench read mapping and Optical mapping developed by OpGen. Over-extension of contigs without prior knowledge of contig location can lead to misassembled contigs, even when commonly used quality indicators such as read mapping suggest that a contig is well assembled. Precautions must also be undertaken when using long read sequencing technology, which may also lead to misassembled contigs. Twit Text FOAVip The hidden perils of read mapping as a quality assessment tool in genome sequencing ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28225089 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28225089 #FOAvip English Wikipedia <ref>{{Cite pmid|28225089}}</ref> The hidden perils of read mapping as a quality assessment tool in genome sequencing #MMPMID28225089 Lehri B; Seddon AM; Karlyshev AV Sci Rep 2017[]; 7 (ä): ä PMID28225089show ga This article provides a comparative analysis of the various methods of genome sequencing focusing on verification of the assembly quality. The results of a comparative assessment of various de novo assembly tools, as well as sequencing technologies, are presented using a recently completed sequence of the genome of Lactobacillus fermentum 3872. In particular, quality of assemblies is assessed by using CLC Genomics Workbench read mapping and Optical mapping developed by OpGen. Over-extension of contigs without prior knowledge of contig location can lead to misassembled contigs, even when commonly used quality indicators such as read mapping suggest that a contig is well assembled. Precautions must also be undertaken when using long read sequencing technology, which may also lead to misassembled contigs. äThe hidden perils of read mapping as a quality assessment tool in geno(epmc).pdf DeepDyve Pubget Overpricing