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10.5483/BMBRep.2017.50.1.128 http://scihub22266oqcxt.onion/10.5483/BMBRep.2017.50.1.128 C5319660!5319660!27616359     free     free     free       BMB+Rep 2017 ; 50 (1): 20-4 Nephropedia Template TP {{tp|p=27616359|t=2017. CRISPR as a strong gene editing tool |pdf=|usr=}}{{27616359}} gab.com Text CRISPR as a strong gene editing tool JO: BMB Rep http://www.kidney.de/pget.php?&tw=1&pmid=27616359 #FOAvip Clustered regularly-interspaced short palindromic repeats (CRISPR) is a new and effective genetic editing tool. CRISPR was initially found in bacteria to protect it from virus invasions. In the first step, specific DNA strands of virus are identified by guide RNA that is composed of crRNA and tracrRNA. Then RNAse III is required for producing crRNA from pre-crRNA. In The second step, a crRNA:tracrRNA:Cas9 complex guides RNase III to cleave target DNA. After cleavage of DNA by CRISPR-Cas9, DNA can be fixed by Non-Homologous End Joining (NHEJ) and Homology Directed Repair (HDR). Whereas NHEJ is simple and random, HDR is much more complex and accurate. Gene editing by CRISPR is able to be applied to various biological field such as agriculture and treating genetic diseases in human. Twit Text FOAVip CRISPR as a strong gene editing tool ????BMB Rep http://www.kidney.de/pget.php?&tw=1&pmid=27616359 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27616359 #FOAvip English Wikipedia <ref>{{Cite pmid|27616359}}</ref> CRISPR as a strong gene editing tool #MMPMID27616359 Shen S; Loh TJ; Shen H; Zheng X; Shen H BMB Rep 2017[]; 50 (1): 20-4 PMID27616359show ga Clustered regularly-interspaced short palindromic repeats (CRISPR) is a new and effective genetic editing tool. CRISPR was initially found in bacteria to protect it from virus invasions. In the first step, specific DNA strands of virus are identified by guide RNA that is composed of crRNA and tracrRNA. Then RNAse III is required for producing crRNA from pre-crRNA. In The second step, a crRNA:tracrRNA:Cas9 complex guides RNase III to cleave target DNA. After cleavage of DNA by CRISPR-Cas9, DNA can be fixed by Non-Homologous End Joining (NHEJ) and Homology Directed Repair (HDR). Whereas NHEJ is simple and random, HDR is much more complex and accurate. Gene editing by CRISPR is able to be applied to various biological field such as agriculture and treating genetic diseases in human. äCRISPR as a strong gene editing tool (epmc).pdf DeepDyve Pubget Overpricing