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10.18632/oncotarget.10979 http://scihub22266oqcxt.onion/10.18632/oncotarget.10979 C5312355!5312355 !27494883     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27494883 &cmd=llinks): Failed to open stream: HTTP request failed! 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Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients |pdf=|usr=}}{{27494883 }} gab.com Text Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27494883 #FOAvip Circulating tumor cells (CTCs) have emerged as promising tools for noninvasive cancer detection and prognosis. Most conventional approaches for capturing CTCs use an EpCAM-based enrichment strategy, which does not work well in cancers that show low or no expression of EpCAM, such as renal cell carcinoma (RCC). In this study, we developed a new set of cell surface markers including CA9 and CD147 as alternative CTC-capture antigens specifically designed for RCC patients. We showed that the expression of both CA9 and CD147 was prevalent in a RCC patient cohort (n=70) by immunohistochemical analysis, with both molecules in combination covering 97.1% of cases. The NanoVelcro platform combined with CA9-/CD147-capture antibodies demonstrated significantly higher efficiency for capturing both CTC-mimicking renal cancer cells and RCC CTCs in peripheral blood, compared to the conventional EpCAM-based method. Using immunofluorescence cytological validation at the single-cell level, we were able to identify bona fide CTCs in RCC patient blood following the well-accepted criteria in our CTC-capture system. We further demonstrated a significant association of CTC numbers as well as the CTC expression status of Vimentin, a mesenchymal marker, with disease progression, including pathologic features and clinical staging. These results provide new insights into developing novel, effective targets/approaches for capturing CTCs, making CTCs a valuable tool for improved cancer detection, prognosis and treatment in RCC. Twit Text FOAVip Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=27494883 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27494883 #FOAvip English Wikipedia <ref>{{Cite pmid|27494883 }}</ref> Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients #MMPMID27494883 Liu S ; Tian Z ; Zhang L ; Hou S ; Hu S ; Wu J ; Jing Y ; Sun H ; Yu F ; Zhao L ; Wang R ; Tseng HR ; Zhau HE ; Chung LW ; Wu K ; Wang H ; Wu JB ; Nie Y ; Shao C Oncotarget 2016[Sep]; 7 (37 ): 59877-59891 PMID27494883 show ga Circulating tumor cells (CTCs) have emerged as promising tools for noninvasive cancer detection and prognosis. Most conventional approaches for capturing CTCs use an EpCAM-based enrichment strategy, which does not work well in cancers that show low or no expression of EpCAM, such as renal cell carcinoma (RCC). In this study, we developed a new set of cell surface markers including CA9 and CD147 as alternative CTC-capture antigens specifically designed for RCC patients. We showed that the expression of both CA9 and CD147 was prevalent in a RCC patient cohort (n=70) by immunohistochemical analysis, with both molecules in combination covering 97.1% of cases. The NanoVelcro platform combined with CA9-/CD147-capture antibodies demonstrated significantly higher efficiency for capturing both CTC-mimicking renal cancer cells and RCC CTCs in peripheral blood, compared to the conventional EpCAM-based method. Using immunofluorescence cytological validation at the single-cell level, we were able to identify bona fide CTCs in RCC patient blood following the well-accepted criteria in our CTC-capture system. We further demonstrated a significant association of CTC numbers as well as the CTC expression status of Vimentin, a mesenchymal marker, with disease progression, including pathologic features and clinical staging. These results provide new insights into developing novel, effective targets/approaches for capturing CTCs, making CTCs a valuable tool for improved cancer detection, prognosis and treatment in RCC. |Adolescent [MESH] |Adult [MESH] |Aged [MESH] |Antibodies/metabolism [MESH] |Antigens, Neoplasm/immunology/*metabolism [MESH] |Basigin/immunology/*metabolism [MESH] |Carbonic Anhydrase IX/immunology/*metabolism [MESH] |Carcinoma, Renal Cell/*diagnosis [MESH] |Cell Adhesion [MESH] |Cohort Studies [MESH] |Female [MESH] |Humans [MESH] |Kidney Neoplasms/*diagnosis [MESH] |Male [MESH] |Middle Aged [MESH] |Neoplastic Cells, Circulating/*metabolism/pathology [MESH] |Prognosis [MESH] |Sensitivity and Specificity [MESH]Combined cell surface carbonic anhydrase 9 and CD147 antigens enable (epmc).pdf DeepDyve Pubget Overpricing