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2017 ; 12
(2
): e0172170
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gab.com Text
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The first initiative of DNA barcoding of ornamental plants from Egypt and
potential applications in horticulture industry
#MMPMID28199378
O Elansary H
; Ashfaq M
; Ali HM
; Yessoufou K
PLoS One
2017[]; 12
(2
): e0172170
PMID28199378
show ga
DNA barcoding relies on short and standardized gene regions to identify species.
The agricultural and horticultural applications of barcoding such as for
marketplace regulation and copyright protection remain poorly explored. This
study examines the effectiveness of the standard plant barcode markers (matK and
rbcL) for the identification of plant species in private and public nurseries in
northern Egypt. These two markers were sequenced from 225 specimens of 161
species and 62 plant families of horticultural importance. The sequence recovery
was similar for rbcL (96.4%) and matK (84%), but the number of specimens assigned
correctly to the respective genera and species was lower for rbcL (75% and 29%)
than matK (85% and 40%). The combination of rbcL and matK brought the number of
correct generic and species assignments to 83.4% and 40%, respectively.
Individually, the efficiency of both markers varied among different plant
families; for example, all palm specimens (Arecaceae) were correctly assigned to
species while only one individual of Asteraceae was correctly assigned to
species. Further, barcodes reliably assigned ornamental horticultural and
medicinal plants correctly to genus while they showed a lower or no success in
assigning these plants to species and cultivars. For future, we recommend the
combination of a complementary barcode (e.g. ITS or trnH-psbA) with rbcL + matK
to increase the performance of taxa identification. By aiding species
identification of horticultural crops and ornamental palms, the analysis of the
barcode regions will have large impact on horticultural industry.