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10.1073/pnas.1617990114 http://scihub22266oqcxt.onion/10.1073/pnas.1617990114 C5293099!5293099 !28096386     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28096386 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2017 ; 114 (5 ): 962-967 Nephropedia Template TP {{tp|p=28096386 |t=2017. Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window |pdf=|usr=}}{{28096386 }} gab.com Text Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=28096386 #FOAvip Fluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-infrared (NIR-I; ?700-900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000-1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ?1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ?170-?m histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II windows (800-1,700 nm). Twit Text FOAVip Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=28096386 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28096386 #FOAvip English Wikipedia <ref>{{Cite pmid|28096386 }}</ref> Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window #MMPMID28096386 Zhu S ; Yang Q ; Antaris AL ; Yue J ; Ma Z ; Wang H ; Huang W ; Wan H ; Wang J ; Diao S ; Zhang B ; Li X ; Zhong Y ; Yu K ; Hong G ; Luo J ; Liang Y ; Dai H Proc Natl Acad Sci U S A 2017[Jan]; 114 (5 ): 962-967 PMID28096386 show ga Fluorescence imaging multiplicity of biological systems is an area of intense focus, currently limited to fluorescence channels in the visible and first near-infrared (NIR-I; ?700-900 nm) spectral regions. The development of conjugatable fluorophores with longer wavelength emission is highly desired to afford more targeting channels, reduce background autofluorescence, and achieve deeper tissue imaging depths. We have developed NIR-II (1,000-1,700 nm) molecular imaging agents with a bright NIR-II fluorophore through high-efficiency click chemistry to specific molecular antibodies. Relying on buoyant density differences during density gradient ultracentrifugation separations, highly pure NIR-II fluorophore-antibody conjugates emitting ?1,100 nm were obtained for use as molecular-specific NIR-II probes. This facilitated 3D staining of ?170-?m histological brain tissues sections on a home-built confocal microscope, demonstrating multicolor molecular imaging across both the NIR-I and NIR-II windows (800-1,700 nm). |*Brain Chemistry [MESH] |*Click Chemistry [MESH] |Animals [MESH] |Biotinylation [MESH] |Brain/*ultrastructure [MESH] |Carcinoma, Squamous Cell/ultrastructure [MESH] |Cetuximab/analysis [MESH] |Fluorescent Antibody Technique, Direct/*methods [MESH] |Fluorescent Dyes/*analysis [MESH] |Imaging, Three-Dimensional [MESH] |Mice [MESH] |Mice, Inbred BALB C [MESH] |Mice, Nude [MESH] |Microscopy, Confocal/methods [MESH] |Molecular Structure [MESH] |Nanotubes [MESH] |Nuclear Magnetic Resonance, Biomolecular [MESH] |Spectroscopy, Near-Infrared/*methods [MESH]Molecular imaging of biological systems with a clickable dye in the br(epmc).pdf DeepDyve Pubget Overpricing