Color-coded Super-resolution Small Molecule Imaging #MMPMID26994590
Beuzer P; La Clair JJ; Cang H
Chembiochem 2016[Jun]; 17 (11): 999-1003 PMID26994590show ga
While the development of super-resolution microscopy dates back to 1994, its applications have been primarily focused on visualizing cellular structures and targets, including proteins, DNA and sugars. We now report on a system that allows one to both monitor the localization of exogenous small molecules in live cells at low resolution and subsequently perform super resolution using stochastic optical reconstruction microscopy (STORM) on fixed cells. This represents a powerful new tool to understand the dynamics of the subcellular trafficking associated with the mode of action (MOA) of exogenous small molecules.