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10.1038/nprot.2016.170 http://scihub22266oqcxt.onion/10.1038/nprot.2016.170 C5278902!5278902!28005067     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28005067&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2017 ; 12 (1): 195-207 Nephropedia Template TP {{tp|p=28005067|t=2017. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells |pdf=|usr=}}{{28005067}} gab.com Text Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28005067 #FOAvip A variety of protocols have been developed which demonstrate a capability to differentiate human pluripotent stem cells (hPSCs) into kidney structures. Our goal was to develop a high efficiency protocol to generate nephron progenitor cells (NPCs) and kidney organoids to facilitate applications for tissue engineering, disease modeling and chemical screening. Here, we describe a detailed protocol resulting in high efficiency production (80?90%) of NPCs within 9 days of differentiation from hPSCs. Kidney organoids were generated from NPCs within 12 days with high reproducibility using 96-well plates suitable for chemical screening. The protocol requires skills in culturing hPSCs and careful attention to morphological changes indicative of differentiation. This kidney organoid system provides a platform for studies of human kidney development, modeling of kidney diseases, nephrotoxicity, and kidney regeneration. The system provides a model for in vitro study of intracellular and kidney inter-compartmental interactions using differentiated human cells in an appropriate nephron and stromal context. Twit Text FOAVip Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28005067 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28005067 #FOAvip English Wikipedia <ref>{{Cite pmid|28005067}}</ref> Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells #MMPMID28005067 Morizane R; Bonventre JV Nat Protoc 2017[Jan]; 12 (1): 195-207 PMID28005067show ga A variety of protocols have been developed which demonstrate a capability to differentiate human pluripotent stem cells (hPSCs) into kidney structures. Our goal was to develop a high efficiency protocol to generate nephron progenitor cells (NPCs) and kidney organoids to facilitate applications for tissue engineering, disease modeling and chemical screening. Here, we describe a detailed protocol resulting in high efficiency production (80?90%) of NPCs within 9 days of differentiation from hPSCs. Kidney organoids were generated from NPCs within 12 days with high reproducibility using 96-well plates suitable for chemical screening. The protocol requires skills in culturing hPSCs and careful attention to morphological changes indicative of differentiation. This kidney organoid system provides a platform for studies of human kidney development, modeling of kidney diseases, nephrotoxicity, and kidney regeneration. The system provides a model for in vitro study of intracellular and kidney inter-compartmental interactions using differentiated human cells in an appropriate nephron and stromal context. äGeneration of nephron progenitor cells and kidney organoids from human(epmc).pdf DeepDyve Pubget Overpricing