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10.1073/pnas.1615735114 http://scihub22266oqcxt.onion/10.1073/pnas.1615735114 C5278437!5278437!28062688     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2017 ; 114 (4): 722-7 Nephropedia Template TP {{tp|p=28062688|t=2017. piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice |pdf=|usr=}}{{28062688}} gab.com Text piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=28062688 #FOAvip Because genome-wide CRISPR/Cas9 libraries are mostly constructed in lentiviral vectors, direct in vivo screening has not been possible as a result of low efficiency in delivery. Here, we examined the piggyBac (PB) transposon as an alternative vehicle to deliver a guide RNA (gRNA) library for in vivo screening. Through hydrodynamic tail vein injections, we delivered a PB-CRISPR library into mouse liver. Rapid tumor formation could be observed in less than 2 mo. By sequencing analysis of PB-mediated gRNA insertions, we identified corresponding genes mediating tumorigenesis. Our results demonstrate that PB is a simple and nonviral choice for efficient in vivo delivery of CRISPR libraries for phenotype-driven screens. Twit Text FOAVip piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=28062688 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28062688 #FOAvip English Wikipedia <ref>{{Cite pmid|28062688}}</ref> piggyBac mediates efficient in vivo CRISPR library screening for tumorigenesis in mice #MMPMID28062688 Xu C; Qi X; Du X; Zou H; Gao F; Feng T; Lu H; Li S; An X; Zhang L; Wu Y; Liu Y; Li N; Capecchi MR; Wu S Proc Natl Acad Sci U S A 2017[Jan]; 114 (4): 722-7 PMID28062688show ga Because genome-wide CRISPR/Cas9 libraries are mostly constructed in lentiviral vectors, direct in vivo screening has not been possible as a result of low efficiency in delivery. Here, we examined the piggyBac (PB) transposon as an alternative vehicle to deliver a guide RNA (gRNA) library for in vivo screening. Through hydrodynamic tail vein injections, we delivered a PB-CRISPR library into mouse liver. Rapid tumor formation could be observed in less than 2 mo. By sequencing analysis of PB-mediated gRNA insertions, we identified corresponding genes mediating tumorigenesis. Our results demonstrate that PB is a simple and nonviral choice for efficient in vivo delivery of CRISPR libraries for phenotype-driven screens. äpiggyBac mediates efficient in vivo CRISPR library screening for tumor(epmc).pdf DeepDyve Pubget Overpricing