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10.1016/j.jaci.2016.04.060 http://scihub22266oqcxt.onion/10.1016/j.jaci.2016.04.060 C5237629!5237629 !27527263     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27527263 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Allergy+Clin+Immunol 2017 ; 139 (3 ): 889-899.e11 Nephropedia Template TP {{tp|p=27527263 |t=2017. Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis |pdf=|usr=}}{{27527263 }} gab.com Text Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis JO: J Allergy Clin Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27527263 #FOAvip BACKGROUND: Basophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies. OBJECTIVE: We attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample. METHODS: Blood from 46 healthy donors and 120 patients with peanut allergy was collected into EDTA or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs. RESULTS: Stimulation with anti-IgE or IL-3 resulted in strong upregulation of basophil CD203c in samples collected in EDTA or heparin, stored at 4°C, and analyzed 24 hours after sample collection. However, a CD63(hi) population of basophils was not observed in any conditions in EDTA-treated samples unless exogenous calcium/magnesium was added at the time of anti-IgE stimulation. By contrast, blood samples collected in heparin tubes were adequate for quantification of upregulation of basophil CD203c and identification of a population of CD63(hi) basophils, irrespective of whether the specimens were analyzed by means of conventional flow cytometry or cytometry by time-of-flight mass spectrometry, and such tests could be performed after blood was stored for 24 hours at 4°C. CONCLUSION: BATs to measure upregulation of basophil CD203c and induction of a CD63(hi) basophil population can be conducted with blood obtained in heparin tubes and stored at 4°C for 24 hours. Twit Text FOAVip Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis ????J Allergy Clin Immunol http://www.kidney.de/pget.php?&tw=1&pmid=27527263 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27527263 #FOAvip English Wikipedia <ref>{{Cite pmid|27527263 }}</ref> Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis #MMPMID27527263 Mukai K ; Gaudenzio N ; Gupta S ; Vivanco N ; Bendall SC ; Maecker HT ; Chinthrajah RS ; Tsai M ; Nadeau KC ; Galli SJ J Allergy Clin Immunol 2017[Mar]; 139 (3 ): 889-899.e11 PMID27527263 show ga BACKGROUND: Basophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies. OBJECTIVE: We attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample. METHODS: Blood from 46 healthy donors and 120 patients with peanut allergy was collected into EDTA or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs. RESULTS: Stimulation with anti-IgE or IL-3 resulted in strong upregulation of basophil CD203c in samples collected in EDTA or heparin, stored at 4°C, and analyzed 24 hours after sample collection. However, a CD63(hi) population of basophils was not observed in any conditions in EDTA-treated samples unless exogenous calcium/magnesium was added at the time of anti-IgE stimulation. By contrast, blood samples collected in heparin tubes were adequate for quantification of upregulation of basophil CD203c and identification of a population of CD63(hi) basophils, irrespective of whether the specimens were analyzed by means of conventional flow cytometry or cytometry by time-of-flight mass spectrometry, and such tests could be performed after blood was stored for 24 hours at 4°C. CONCLUSION: BATs to measure upregulation of basophil CD203c and induction of a CD63(hi) basophil population can be conducted with blood obtained in heparin tubes and stored at 4°C for 24 hours. |Adolescent [MESH] |Adult [MESH] |Antibodies, Anti-Idiotypic/pharmacology [MESH] |Anticoagulants/pharmacology [MESH] |Basophil Degranulation Test/*methods [MESH] |Basophils/drug effects [MESH] |Child [MESH] |Edetic Acid/pharmacology [MESH] |Female [MESH] |Flow Cytometry [MESH] |Heparin/pharmacology [MESH] |Humans [MESH] |Interleukin-3/pharmacology [MESH] |Male [MESH] |Middle Aged [MESH] |Peanut Hypersensitivity/blood/immunology [MESH] |Phosphoric Diester Hydrolases/metabolism [MESH] |Pyrophosphatases/metabolism [MESH] |Specimen Handling/*methods [MESH] |Temperature [MESH] |Time Factors [MESH]Assessing basophil activation by using flow cytometry and mass cytomet(epmc).pdf DeepDyve Pubget Overpricing