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10.1128/JCM.01701-16 http://scihub22266oqcxt.onion/10.1128/JCM.01701-16 C5228265!5228265 !27795341     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27795341 &cmd=llinks): Failed to open stream: HTTP request failed! 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Serology Enhances Molecular Diagnosis of Respiratory Virus Infections Other than Influenza in Children and Adults Hospitalized with Community-Acquired Pneumonia |pdf=|usr=}}{{27795341 }} gab.com Text Serology Enhances Molecular Diagnosis of Respiratory Virus Infections Other than Influenza in Children and Adults Hospitalized with Community-Acquired Pneumonia JO: J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27795341 #FOAvip Both molecular and serological assays have been used previously to determine the etiology of community-acquired pneumonia (CAP). However, the extent to which these methods are correlated and the added diagnostic value of serology for respiratory viruses other than influenza virus have not been fully evaluated. Using data from patients enrolled in the Centers for Disease Control and Prevention (CDC) Etiology of Pneumonia in the Community (EPIC) study, we compared real-time reverse transcription-PCR (RT-PCR) and serology for the diagnosis of respiratory syncytial virus (RSV), human metapneumovirus (HMPV), parainfluenza virus 1 to 3 (PIV1, PIV2, and PIV3), and adenovirus (AdV) infections. Of 5,126 patients enrolled, RT-PCR and serology test results were available for 2,023, including 1,087 children below the age of 18 years and 936 adults. For RSV, 287 (14.2%) patients were positive by RT-PCR and 234 (11.6%) were positive by serology; for HMPV, 172 (8.5%) tested positive by RT-PCR and 147 (7.3%) by serology; for the PIVs, 94 (4.6%) tested positive by RT-PCR and 92 (4.6%) by serology; and for AdV, 111 (5.5%) tested positive by RT-PCR and 62 (3.1%) by serology. RT-PCR provided the highest number of positive detections overall, but serology increased diagnostic yield for RSV (by 11.8%), HMPV (by 25.0%), AdV (by 32.4%), and PIV (by 48.9%). The method concordance estimated by Cohen's kappa coefficient (?) ranged from good (for RSV; ? = 0.73) to fair (for AdV; ? = 0.27). Heterotypic seroresponses observed between PIVs and persistent low-level AdV shedding may account for the higher method discordance observed with each of these viruses. Serology can be a helpful adjunct to RT-PCR for research-based assessment of the etiologic contribution of respiratory viruses other than influenza virus to CAP. Twit Text FOAVip Serology Enhances Molecular Diagnosis of Respiratory Virus Infections Other than Influenza in Children and Adults Hospitalized with Community-Acquired Pneumonia ????J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=27795341 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27795341 #FOAvip English Wikipedia <ref>{{Cite pmid|27795341 }}</ref> Serology Enhances Molecular Diagnosis of Respiratory Virus Infections Other than Influenza in Children and Adults Hospitalized with Community-Acquired Pneumonia #MMPMID27795341 Zhang Y ; Sakthivel SK ; Bramley A ; Jain S ; Haynes A ; Chappell JD ; Hymas W ; Lenny N ; Patel A ; Qi C ; Ampofo K ; Arnold SR ; Self WH ; Williams DJ ; Hillyard D ; Anderson EJ ; Grijalva CG ; Zhu Y ; Wunderink RG ; Edwards KM ; Pavia AT ; McCullers JA ; Erdman DD J Clin Microbiol 2017[Jan]; 55 (1 ): 79-89 PMID27795341 show ga Both molecular and serological assays have been used previously to determine the etiology of community-acquired pneumonia (CAP). However, the extent to which these methods are correlated and the added diagnostic value of serology for respiratory viruses other than influenza virus have not been fully evaluated. Using data from patients enrolled in the Centers for Disease Control and Prevention (CDC) Etiology of Pneumonia in the Community (EPIC) study, we compared real-time reverse transcription-PCR (RT-PCR) and serology for the diagnosis of respiratory syncytial virus (RSV), human metapneumovirus (HMPV), parainfluenza virus 1 to 3 (PIV1, PIV2, and PIV3), and adenovirus (AdV) infections. Of 5,126 patients enrolled, RT-PCR and serology test results were available for 2,023, including 1,087 children below the age of 18 years and 936 adults. For RSV, 287 (14.2%) patients were positive by RT-PCR and 234 (11.6%) were positive by serology; for HMPV, 172 (8.5%) tested positive by RT-PCR and 147 (7.3%) by serology; for the PIVs, 94 (4.6%) tested positive by RT-PCR and 92 (4.6%) by serology; and for AdV, 111 (5.5%) tested positive by RT-PCR and 62 (3.1%) by serology. RT-PCR provided the highest number of positive detections overall, but serology increased diagnostic yield for RSV (by 11.8%), HMPV (by 25.0%), AdV (by 32.4%), and PIV (by 48.9%). The method concordance estimated by Cohen's kappa coefficient (?) ranged from good (for RSV; ? = 0.73) to fair (for AdV; ? = 0.27). Heterotypic seroresponses observed between PIVs and persistent low-level AdV shedding may account for the higher method discordance observed with each of these viruses. Serology can be a helpful adjunct to RT-PCR for research-based assessment of the etiologic contribution of respiratory viruses other than influenza virus to CAP. |Adolescent [MESH] |Adult [MESH] |Aged [MESH] |Aged, 80 and over [MESH] |Child [MESH] |Child, Preschool [MESH] |Community-Acquired Infections/*diagnosis [MESH] |Female [MESH] |Hospitalization [MESH] |Humans [MESH] |Infant [MESH] |Infant, Newborn [MESH] |Male [MESH] |Middle Aged [MESH] |Molecular Diagnostic Techniques/*methods [MESH] |Pneumonia, Viral/*diagnosis [MESH] |Real-Time Polymerase Chain Reaction/methods [MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods [MESH] |Sensitivity and Specificity [MESH] |Serologic Tests/*methods [MESH]Serology Enhances Molecular Diagnosis of Respiratory Virus Infections (epmc).pdf DeepDyve Pubget Overpricing