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10.1038/nprot.2016.105 http://scihub22266oqcxt.onion/10.1038/nprot.2016.105 C5223290!5223290 !27560178     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27560178 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Protoc 2016 ; 11 (9 ): 1757-74 Nephropedia Template TP {{tp|p=27560178 |t=2016. Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes |pdf=|usr=}}{{27560178 }} gab.com Text Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=27560178 #FOAvip In morphological profiling, quantitative data are extracted from microscopy images of cells to identify biologically relevant similarities and differences among samples based on these profiles. This protocol describes the design and execution of experiments using Cell Painting, which is a morphological profiling assay that multiplexes six fluorescent dyes, imaged in five channels, to reveal eight broadly relevant cellular components or organelles. Cells are plated in multiwell plates, perturbed with the treatments to be tested, stained, fixed, and imaged on a high-throughput microscope. Next, an automated image analysis software identifies individual cells and measures ?1,500 morphological features (various measures of size, shape, texture, intensity, and so on) to produce a rich profile that is suitable for the detection of subtle phenotypes. Profiles of cell populations treated with different experimental perturbations can be compared to suit many goals, such as identifying the phenotypic impact of chemical or genetic perturbations, grouping compounds and/or genes into functional pathways, and identifying signatures of disease. Cell culture and image acquisition takes 2 weeks; feature extraction and data analysis take an additional 1-2 weeks. Twit Text FOAVip Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=27560178 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27560178 #FOAvip English Wikipedia <ref>{{Cite pmid|27560178 }}</ref> Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes #MMPMID27560178 Bray MA ; Singh S ; Han H ; Davis CT ; Borgeson B ; Hartland C ; Kost-Alimova M ; Gustafsdottir SM ; Gibson CC ; Carpenter AE Nat Protoc 2016[Sep]; 11 (9 ): 1757-74 PMID27560178 show ga In morphological profiling, quantitative data are extracted from microscopy images of cells to identify biologically relevant similarities and differences among samples based on these profiles. This protocol describes the design and execution of experiments using Cell Painting, which is a morphological profiling assay that multiplexes six fluorescent dyes, imaged in five channels, to reveal eight broadly relevant cellular components or organelles. Cells are plated in multiwell plates, perturbed with the treatments to be tested, stained, fixed, and imaged on a high-throughput microscope. Next, an automated image analysis software identifies individual cells and measures ?1,500 morphological features (various measures of size, shape, texture, intensity, and so on) to produce a rich profile that is suitable for the detection of subtle phenotypes. Profiles of cell populations treated with different experimental perturbations can be compared to suit many goals, such as identifying the phenotypic impact of chemical or genetic perturbations, grouping compounds and/or genes into functional pathways, and identifying signatures of disease. Cell culture and image acquisition takes 2 weeks; feature extraction and data analysis take an additional 1-2 weeks. |Cell Line, Tumor [MESH] |Cell Shape [MESH] |Cell Size [MESH] |Fluorescent Dyes/*metabolism [MESH] |Humans [MESH] |Image Processing, Computer-Assisted [MESH] |Molecular Imaging/*methods [MESH]Cell Painting, a high-content image-based assay for morphological prof(epmc).pdf DeepDyve Pubget Overpricing