Genetic code expansion in the mouse brain #MMPMID27571478
Ernst RJ; Krogager TP; Maywood ES; Zanchi R; Beránek V; Elliott TS; Barry NP; Hastings MH; Chin JW
Nat Chem Biol 2016[Oct]; 12 (10): 776-8 PMID27571478show ga
Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNAPylCUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice.