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10.1016/j.bbamcr.2016.07.008 http://scihub22266oqcxt.onion/10.1016/j.bbamcr.2016.07.008 C5206903!5206903 !27496272     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27496272 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biochim+Biophys+Acta 2016 ; 1863 (11 ): 2624-2636 Nephropedia Template TP {{tp|p=27496272 |t=2016. Low dose ouabain stimulates NaK ATPase ?1 subunit association with angiotensin II type 1 receptor in renal proximal tubule cells |pdf=|usr=}}{{27496272 }} gab.com Text Low dose ouabain stimulates NaK ATPase 1 subunit association with angiotensin II type 1 receptor in renal proximal tubule cells JO: Biochim Biophys Acta http://www.kidney.de/pget.php?&tw=1&pmid=27496272 #FOAvip Our laboratory has recently demonstrated that low concentrations of ouabain increase blood pressure in rats associated with stimulation of NaK ATPase activity and activation of the Src signaling cascade in NHE1-dependent manner. Proteomic analysis of human kidney proximal tubule cells (HKC11) suggested that the Angiotensin II type 1 receptor (AT1R) as an ouabain-associating protein. We hypothesize that ouabain-induced stimulation of NaK ATPase activity is mediated through AT1R. To test this hypothesis, we examined the effect of ouabain on renal cell angiotensin II production, the effect of AT1R inhibition on ouabain-stimulated NKA activity, and the effect of ouabain on NKA-AT1R association. Ouabain increased plasma angiotensin II levels in rats treated with ouabain (1?g/kg body wt./day) for 9days and increased angiotensin II levels in cell culture media after 24h treatment with ouabain in human (HKC11), mouse (MRPT), and human adrenal cells. Ouabain 10pM stimulated NKA-mediated (86)Rb uptake and phosphorylation of EGFR, Src, and ERK1/2. These effects were prevented by the AT1R receptor blocker candesartan. FRET and TIRF microscopy using Bodipy-labeled ouabain and mCherry-NKA or mCherry-AT1R demonstrated association of ouabain with AT1R and NKA. Further our FRET and TIRF studies demonstrated increased association between AT1R and NKA upon treatment with low dose ouabain. We conclude that ouabain stimulates NKA in renal proximal tubule cells through an angiotensin/AT1R-dependent mechanism and that this pathway contributes to cardiac glycoside associated hypertension. Twit Text FOAVip Low dose ouabain stimulates NaK ATPase 1 subunit association with angiotensin II type 1 receptor in renal proximal tubule cells ????Biochim Biophys Acta http://www.kidney.de/pget.php?&tw=1&pmid=27496272 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27496272 #FOAvip English Wikipedia <ref>{{Cite pmid|27496272 }}</ref> Low dose ouabain stimulates NaK ATPase ?1 subunit association with angiotensin II type 1 receptor in renal proximal tubule cells #MMPMID27496272 Ketchem CJ ; Conner CD ; Murray RD ; DuPlessis M ; Lederer ED ; Wilkey D ; Merchant M ; Khundmiri SJ Biochim Biophys Acta 2016[Nov]; 1863 (11 ): 2624-2636 PMID27496272 show ga Our laboratory has recently demonstrated that low concentrations of ouabain increase blood pressure in rats associated with stimulation of NaK ATPase activity and activation of the Src signaling cascade in NHE1-dependent manner. Proteomic analysis of human kidney proximal tubule cells (HKC11) suggested that the Angiotensin II type 1 receptor (AT1R) as an ouabain-associating protein. We hypothesize that ouabain-induced stimulation of NaK ATPase activity is mediated through AT1R. To test this hypothesis, we examined the effect of ouabain on renal cell angiotensin II production, the effect of AT1R inhibition on ouabain-stimulated NKA activity, and the effect of ouabain on NKA-AT1R association. Ouabain increased plasma angiotensin II levels in rats treated with ouabain (1?g/kg body wt./day) for 9days and increased angiotensin II levels in cell culture media after 24h treatment with ouabain in human (HKC11), mouse (MRPT), and human adrenal cells. Ouabain 10pM stimulated NKA-mediated (86)Rb uptake and phosphorylation of EGFR, Src, and ERK1/2. These effects were prevented by the AT1R receptor blocker candesartan. FRET and TIRF microscopy using Bodipy-labeled ouabain and mCherry-NKA or mCherry-AT1R demonstrated association of ouabain with AT1R and NKA. Further our FRET and TIRF studies demonstrated increased association between AT1R and NKA upon treatment with low dose ouabain. We conclude that ouabain stimulates NKA in renal proximal tubule cells through an angiotensin/AT1R-dependent mechanism and that this pathway contributes to cardiac glycoside associated hypertension. |Angiotensin II Type 1 Receptor Blockers/pharmacology [MESH] |Angiotensin II/metabolism [MESH] |Angiotensinogen/metabolism [MESH] |Animals [MESH] |Cell Line [MESH] |Dose-Response Relationship, Drug [MESH] |Enzyme Activation [MESH] |Enzyme Activators/*pharmacology/toxicity [MESH] |Hypertension/chemically induced/enzymology [MESH] |Kidney Tubules, Proximal/*drug effects/enzymology [MESH] |Mice [MESH] |Ouabain/*pharmacology/toxicity [MESH] |Peptidyl-Dipeptidase A/metabolism [MESH] |Phosphorylation [MESH] |Protein Binding [MESH] |Rats, Sprague-Dawley [MESH] |Receptor, Angiotensin, Type 1/genetics/*metabolism [MESH] |Signal Transduction/drug effects [MESH] |Sodium-Potassium-Exchanging ATPase/genetics/*metabolism [MESH]Low dose ouabain stimulates NaK ATPase ?1 subunit association with ang(epmc).pdf DeepDyve Pubget Overpricing