Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27930297
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Proc+Natl+Acad+Sci+U+S+A
2016 ; 113
(51
): E8219-E8227
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Measuring spectroscopy and magnetism of extracted and intracellular magnetosomes
using soft X-ray ptychography
#MMPMID27930297
Zhu X
; Hitchcock AP
; Bazylinski DA
; Denes P
; Joseph J
; Lins U
; Marchesini S
; Shiu HW
; Tyliszczak T
; Shapiro DA
Proc Natl Acad Sci U S A
2016[Dec]; 113
(51
): E8219-E8227
PMID27930297
show ga
Characterizing the chemistry and magnetism of magnetotactic bacteria (MTB) is an
important aspect of understanding the biomineralization mechanism and function of
the chains of magnetosomes (Fe(3)O(4) nanoparticles) found in such species.
Images and X-ray absorption spectra (XAS) of magnetosomes extracted from, and
magnetosomes in, whole Magnetovibrio blakemorei strain MV-1 cells have been
recorded using soft X-ray ptychography at the Fe 2p edge. A spatial resolution of
7 nm is demonstrated. Precursor-like and immature magnetosome phases in a whole
MV-1 cell were visualized, and their Fe 2p spectra were measured. Based on these
results, a model for the pathway of magnetosome biomineralization for MV-1 is
proposed. Fe 2p X-ray magnetic circular dichroism (XMCD) spectra have been
derived from ptychography image sequences recorded using left and right circular
polarization. The shape of the XAS and XMCD signals in the ptychographic
absorption spectra of both sample types is identical to the shape and signals
measured with conventional bright-field scanning transmission X-ray microscope. A
weaker and inverted XMCD signal was observed in the ptychographic phase spectra
of the extracted magnetosomes. The XMCD ptychographic phase spectrum of the
intracellular magnetosomes differed from the ptychographic phase spectrum of the
extracted magnetosomes. These results demonstrate that spectro-ptychography
offers a superior means of characterizing the chemical and magnetic properties of
MTB at the individual magnetosome level.